This study aims to determine the potential of Bacillus subtilis probiotic in reducing levels of ammonia, Vibrio sp, and increased production performance in seaworm cultivation (Nereis sp.) under laboratory scale. Observation of the performance of seaworms (Nereis sp.) was carried out every 10 days which included weight gain (gr), length (cm), total biomass (gr), total bacteria (CFU/mL), total Vibrio sp. (CFU/mL), and total Bacillus subtilis (CFU/mL). Water quality measurements include temperature, dissolved oxygen (DO), pH, ammonia, and total organic matter (TOM). Application of probiotics Bacillus subtilis has the potential to reduce ammonia concentration, increase growth, and reduce the abundance of Vibrio sp under laboratory-scale seaworm cultivation. P3 treatment (0.01 mL with a probiotic density of 10 6 CFU/mL) gave the best results by being able to reduce the ammonia concentration by 47.5%. In summary, the probiotic application using the bacteria Bacillus subtilis with different densities able to provide good results in supporting production performance, maintaining the abundance of Vibrio sp., and reducing ammonia concentration in seaworm cultivation. This is the first study to report the performance of seaworm production using probiotic agent, research is still needed to determine the digestive enzyme activity of seaworms given probiotics.
Ikan lele merupakan sumber protein hewani yang bernilai ekonomis dan kebutuhannya terus meningkat setiap tahun. Keunggulan teknologi bioflok yang mampu menggenjot produktivitas ikan menjadi lebih tinggi dengan penggunaan lahan yang tidak terlalu luas. Tujuan penelitian adalah mengevaluasi teknis budidaya ikan lele sistem bioflok, implementasi intervensi untuk meningkatkan produktivitas budidaya ikan lele sistem bioflok, mengevaluasi performa kinerja budidaya ikan lele sistem bioflok setelah implementasi intervensi, menganalisis finansial budidaya ikan lele sistem bioflok. Hasil penelitian menunjukkan rata-rata performansi kinerja budidaya dan produktivitas mengalami peningkatan, sedangkan, rasio konversi pakan mengalami kenaikan. Intervensi berdasarkan prioritas usulan pemecahan masalah yakni penambahan kuantitas blower dan titik aerasi, pembuatan water level, sampling mingguan dan membeli benih bersertifikat. Implementasi yang dilakukan mampu meningkatkan dari produktivitas sebesar Rp526.687 menjadi sebesar Rp1.242.187 dengan tingkat kelangsungan hidup sebesar 71%. Berdasarkan hasil analisis finansial layak untuk dilanjutkan dengan R/C Ratio sebesar 1,06.
Lele sangkuriang (Clarias gariepinus) merupakan varietas ikan lele unggul dan bernilai ekonomi tinggi. Pakan awal yang baik bagi larva ikan lele sangkuriang adalah pakan alami, seperti cacing sutra. Saat ini penyediaan cacing sutra masih banyak mengandalkan dari alam. Penelitian ini bertujuan menganalisis kandungan nutrisi, kandungan bakteri dan kinerja pakan alami cacing sutra dari tiga sumber berbeda merujuk pada pertumbuhan dan kelangsungan hidup larva ikan lele sangkuriang (Clarias gariepinus). Metode yang digunakan adalah eksperimen dengan Rancangan Acak Lengkap (RAL) dengan variabel uji berupa sumber pakan yang berbeda, di antaranya perlakuan A (cacing sutra hasil pemeliharaan pada media campuran ampas tahu 80%, dedak padi 10%, dan tepung ikan 10%), perlakuan B (cacing sutra hasil budidaya petani) dan perlakuan C (cacing sutra hasil tangkapan dari alam). Hasil penelitian kandungan nutrisi masing-masing perlakuan kandungan protein tertinggi terdapat pada perlakuan C sebesar 11,10%, kemudian perlakuan A sebesar 10,98%, dan terkecil pada perlakuan B yaitu sebesar10,23%. Hasil uji kandungan bakteri cacing sutra pada perlakuan A tidak mengandung bakteri Salmonella sp. maupun bakteri Escherichia coli, pada perlakuan B dan perlakuan C positif mengandung bakteri Escherichia coli. Perlakuan A memberikan tingkat efektivitas terbaik terhadap SR yaitu sebesar 87±0,007c %, pertumbuhan panjang mutlak sebesar 40,07±1,87c mm, berat mutlak sebesar 0,4701±0,0836 g dan rasio konversi pakan sebesar 3,740±0,6189 g. Hasil uji ANOVA menunjukkan perlakuan yang diberikan berpengaruh signifikan (P0,05) terhadap pertumbuhan berat mutlak, SGR, dan rasio konversi pakan.
A prototype design for domestic wastewater treatment has been carried out using the concept of filtration, in which this prototype consists of 2 filters to treat domestic wastewater, the first filter uses a 10-inch cartridge filter media in which filter materials are composed, namely cotton, ferrite, and activated carbon. Then the second filter uses an RO 75 GPD filter which consists of a hollow fiber membrane and has pores of 0.0001 microns. Domestic wastewater from the input reservoir is pumped through the PVC and into filter 1, namely the carbon filter media inside the cartridge filter, the produced water is passed through the ferrite filtration media and then inserted into the membrane, the produced water enters the membrane and RO filter then produces processed wastewater that has been clean and enter into the output reservoir. The results of the functional test explained that the flow velocity of treated wastewater passing through the ¾ inch connecting pipe was 0.22 m/s or 0.24 m3/hour and it took 2 hours to fill an aquarium with a water capacity of 57 liters.
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