Les substances naturelles issues des végétaux présen-tent des intérêts multiples mis à profit dans la biotechnologie tant dans l'industrie alimentaire, cosmétique que pharmaceutique. Parmi ces composés, on retrouve une grande partie des métabolites secondaires qui se sont illustrés dans beaucoup de domaines et même en thérapie. Dans ce contexte, nous avons recherché l'activité antioxydante de Berberis vulgaris, une plante utilisée en Algérie et plus particulièrement dans la région de Tlemcen. Après préparation des extraits à partir des écorces de racines de Berberis vulgaris, les alcaloïdes totaux ont été analysés par chromatographie sur couche mince, révélant la présence de six taches. Aussi, une analyse quantitative des polyphénols et des flavonoïdes de l'extrait hydrométhanolique a été réalisée, montrant une teneur de 10,48 mg GAE/g en polyphénols totaux et 2,05 mg CEQ/g en flavonoïdes. L'activité antioxydante des extraits hydrométhanolique et alcaloïdes totaux a été évaluée par deux méthodes : la réduction du fer et le piégeage du radical libre DPPH. Les résultats obtenus ont montré une activité élevée de l'extrait hydrométhanolique par rapport aux alcaloïdes totaux, avec une CI 50 de 1,40 mg/ml. Mots clés : Berberis vulgaris -Extrait hydrométhanolique -Alcaloïdes totaux -Activité antioxydanteEvaluation of the Antioxidant Activity of Berberis vulgaris L.Abstract: Natural substances from plants have multiple interests exploited in biotechnology industry both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites that are illustrated in many areas and even in therapy. In this context, we investigated the antioxidant activity of Berberis vulgaris, a plant used in Algeria, and it is particularly used in the Tlemcen region. After preparation of extracts from the root bark of Berberis vulgaris, total alkaloids were analyzed by thin layer chromatography, indicating the presence of six tasks. A quantitative analysis of polyphenols and flavonoids in methanolic extract was carried out, showing a content of 10.48 mg GAE/g of total polyphenols and 2.05 mg CEQ/g of flavonoids. The antioxidant activity of hydromethanolic extracts under reflux and total alkaloids was evaluated using two methods: reducing power and DPPH free radical scavenging. The results showed a high activity of methanolic extract than the total alkaloids, with an IC 50 of 1.40 mg/ml.
Evaluation of medicinal plants for their antidiabetic activities has increased considerably around the world as well as in Algeria. Olea europaea var. sylvestris, commonly called Ezzeboudj, is traditionally used by Tlemcen population in Algeria, for treating diabetes mellitus. In this work, we studied the antioxidant activity of wild olive leaves crude extracts, by free radical DPPH scavenging method and iron reduction FRAP. On the other hand, evaluation of the ability of the extracts to inhibit α-amylase activity. The qualitative phytochemical screening carried out on O. europaea var. sylvestris leaves extracts showed the presence of tannins, sterols and triterpenes, saponins, flavonoids and terpenoids. A quantitative analysis of the crude extracts showed significant levels of total polyphenols and flavonoids in hydroacetonic extract, with an amount of 228,45 ± 0,03 mg GAE /g and 204,8 ± 0,04 mg CE /g, respectively. The results obtained from the antioxidant activity showed a high scavenging activity for hydroacetonic extract with an IC50 of 7,95 ± 0,16 µg/ml. In vitro tests carried out on the inhibitory of α-amylase activity, revealed an inhibitory effects, specifically with hydromethanolic extract in a concetration-dependent manner, with an IC50 value of 0,91 ± 0,02 mg/ml. These findings reveals that O. europaea var. sylvestris leaves could represent an interesting source of antioxidants and allow to the development of new antidiabetic agents.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.