In this study, bioactive compounds, oil, sugar, fatty acid, and mineral contents of grape wastes (pomace, skin, and seeds) obtained from wine, grape juice, and boilled grape juice production were investigated. Total phenol and tannin contents of grape by‐products varied between 31.2 mgGAE/g (molasses skin) and 98.97 mgGAE/g (wine seed); 96.93 mgTAE/g (grape juice pomace) and 138.67 mgTAE/g (molasses pomace), respectively. The highest (377.57 g/kg) and lowest (20.00 g/kg) total sugars were determined in molasses and wine skin wastes, respectively. Epicatechin contents of samples were found between 439.67 mg/kg (molasses skin) and 3,444.57 mg/kg (molasses seed). The lowest and highest linoleic acids were determined in molasses skin oil (40.00%) and grape juice skin oil (51.10%). α‐Tocopherol contents of wine by‐product oils changed between 3.35 mg/kg (seed) and 6.42 mg/kg (pomace). The lowest and highest P contents were determined in molasses skin (17,563 mg/kg) and wine seed (29,634 mg/kg), respectively.
Practical applications
The residue may represent from 13.5 to 14.5% at the total volume of grapes, and may reach 20%. The most abundant phenolic compound in wine pomace is anthocyanins concentrated in the skin, and flavonols present mostly in the grape seed (56–65% total flavonol). Grape is a phenol‐rich plant, and these phenolics are mainly distributed in the skin, stem, leaf, and seed of grape, rather than their juicy middle sections. Skins and seeds of grapes are produced in large quantities by the winemaking industry. These by‐products have become valuable raw materials due to their high content of polyphenols, tocols, and other macro‐ and micronutrients. Seed and skins of grape produced in large quantities by the wine making industry have become valuable raw materials for extraction of polyphenols.
Bee pollen and propolis were collected from Apis mellifera colonies in five regions of Turkey. The antifungal properties of methanol extracts of pollen and propolis (2% and 5% concentrations) were determined on Alternaria alternata and Fusarium oxysporium f. sp. melonis. The least active concentration towards the tested fungi was 2% concentration of both extracts. The inhibitory effect of all propolis extracts on growth of F. oxysporium and A. alternata were generally higher when compared with pollen extracts. The growth of A. alternata and F. oxysporium were not affected at both concentrations of pollens. However, F. oxysporium against propolis extracts was more sensitive than A. alternata (P < 0.01). None of the pollen extracts tested completely inhibited mycelial growth of fungi used in our experiment. Percent inhibition of both pollen concentrations against A. alternata and F. oxysporium was lower than 50%. However, both concentrations of Alanya and Beyşehir propolis extracts were 100% effective on mycelial growth of F. oxysporium until the 7th day of incubation (P < 0.01). 2% Alanya and Beyşehir pollen extracts completely stimulated mycelial growth of F. oxysporium on the 7th day of incubation. Both concentrations of propolis extract showed more than 50% inhibition against E. oxysporium. It is suggested that high concentrations ofpropolis extract could be used as an antifungal agent against tested fungi.
The objective of the present work was to investigate the influence of locations on bioactive propertiest, phenolic compounds and mineral contents of bee pollens. The oil content of pollen grains changed between 3.50% (Alanya) and 6.85% (Russia-Perm Region). The highest total phenolic content (720 mg/100g) and antioxidant activity values (81.4%) were observed in pollens obtained from the Russia-Perm Region and Alanya districts, respectively. Additionally, the highest carotenoid was found in a pollen sample collected from Karaman (Sarıveliler) (98.6 mg/g). The major phenolic compounds were (+)-catechin (66.75-337.39 mg/100g) and quercetin (61.2-1221.7 mg/100g) in all pollen samples. The pollen samples were observed to be a significant source of potassium (3846-6287 mg/kg), phosphorus (2947-5010 mg/kg), calcium (1022-2424 mg/kg) and sulfur (1744-2397 mg/kg). All of the analysis results were significantly affected by supplying locations. The antioxidant activity values of pollens were found partly similar and varied depending on locations. The content of saturated fatty acid (palmitic) was high (20-30%) in the tested pollen samples but did not exceed the content of linoleic acid.
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