To investigate the expression of High Mobility Group Box 1 (HMGB1) and Heat Shock Protein-70 (HSP-70) during orthodontic tooth movement (OTM) after (-)-Epigallocatechin-3-Gallate (EGCG) in East Java Green Tea (Camelia Sinensis) Methanolic Extract (GTME) administration in vivo. Material and Methods: 28 Wistar rats (Rattus Novergicus) was used and divided into 4 groups accordingly: K-without EGCG and OTM; K+ with OTM, without EGCG for 14 days; T1with OTM for 14 days and EGCG for 7 days; treatment group 2 (T2) with OTM and EGCG for 14 days. OTM animal model was achieved through the installation of the OTM device by means of NiTi close coil spring with 10g force placed between the first incisor and first maxillary molars. The samples were terminated on Day 14. The pre-maxillary was isolated for the immunohistochemical examination. Analysis of Variance (ANOVA) then continued with Tukey Honest Significant Difference (HSD) (p<0.05) was performed to analyze the data. Results: The highest HMGB1 and HSP-70 expression were found in the K+ group pressure side, meanwhile the lowest HMGB1 and HSP-70 expression were found in K-group tension side in the alveolar bone. There was a significant decrease of HMGB1 and HSP-70 expression in T2 compared to T1 and K+ with significant between groups (p<0.05; p=0.0001). Conclusion: The decreased expression of HMGB1 and HSP-70 in alveolar bone of OTM wistar rats due to post administration of GTME that consisted EGCG.

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The survival of <em>Aedes aegypti</em> larvae is inseparable from the adequacy of food, including organic substances available in the breeding water. It is very dependent on the level of water markers such as temperature, salinity, Dissolved Oxygen, and pH. The study used quantitative observational analytic with a case-control study design. Case group has consisted of breeding water in endemic area and control group was in non-endemic area. The sample size was 43 samples for each group, collected by purposive sampling technique. Data were analyzed by Chi-square and Mann-Whitney test. Larvae mostly presence in endemic area (68.3%) and mostly absent in non-endemic area (85.4%) (<em>p</em>-value = 0.002). Temperature in endemic area mostly in 27-30^oC (86%) and non-endemic area mostly in &lt;27^oC or &gt;30^oC (72.1%) (<em>p</em>-value = 0.000). Salinity in endemic and non-endemic areas has no difference (<em>p</em>-value = 0.266). DO in endemic areas were mostly in 5.02-7.82 mg/l (76.7%). While DO in non-endemic area was mostly in &lt;5.02 mg/l or &gt;7.82 mg/l (95.3%) (<em>p</em>-value = 0.001). The pH &lt;6 or &gt;7.8 is mostly in non-endemic areas (87.8%) and pH 6-7.8 is mostly in endemic areas (63.4%) (<em>p</em>-value = 0.000). Bio-physicochemical markers of breeding sites water have differences between endemic and non-endemic area except salinity. The temperature, salinity, DO, and pH affected the presence of larvae and the most affected is DO marker. While the marker that affected the presence of larvae in the non-endemic area is pH.

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The study aims to determine differences in water quality of breeding sites in endemic and non-endemic areas. The method used is quantitative observational analytic with a case control study design. Case group was water parameters in endemic areas and the control group is in non-endemic areas. 43 samples of breeding water were taken from each area then water quality measurements were carried out. Data were analyzed using independent t-test. The results obtained mean temperature in endemic areas 27.51 ± 0.739 oC, salinity 2,544 ± 0.638 gr/l, and DO 7,253 ± 1,097 mg/l. The mean temperature in non-endemic areas is 25.7 ± 1.124 oC, salinity is 2.472 ± 2.365 gr/l, and DO is 6.479 ± 1.059 mg/l. P-value of statistical tests of differences in temperature, salinity, and DO parameters in endemic and non-endemic areas are 0.000, 0.266, and 0.001. It was concluded that temperature and DO parameters in endemic areas proved to be significantly different from those in non-endemic areas. However, for salinity variables there are no significant differences. Keywords: Aedes aegypti, dissolved oxygen, salinity, temperature