OBJECTIVES:To establish the validity and reliability of imprint cytology and its accuracy in intra operative diagnosis of ovarian tumours and to compare it with the histopathology reports. METHODS: Multiple imprints were taken from 30 surgical specimens of patients suspected of ovarian neoplasms who underwent surgery as primary line therapy at our institution. This was compared with Final pathological diagnoses. We calculated the limits among benign, borderline, and malignant lesions, and analyzed the diagnostic accuracy. RESULTS: 30 cases were studied during this study period from November 2011 to October 2013. 19 (63.3%) cases were benign, 8 cases were malignant (26.7%), 1 (3.3%) case was borderline malignancy and 2 (6.7%) cases were excluded. Overall diagnostic accuracy was 96.4%. CONCLUSION: Imprint cytology is a less expensive, simple and quick method of diagnosis, and is reliable in terms of accuracy to aid decision making intraoperatively.