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An alternative bulk extraction method has been developed for the isolation of sporidesmin from ryecorn cultures ofPithomyces chartarum (Berk. & Curt.) M. B. Ellis. Based on 32 consecutive extractions, an average yield of 165.5 mg sporidesmin-benzene solvate per kg of culture material was achieved, representing a 28-65% increase in yield over previously published results. Furthermore, the extraction process is rapid and can be conducted under normal laboratory conditions without significant loss of sporidesmin through degradation.

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