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The relation of nutritional state to certain toxic manifestations caused by carbon tetrachloride (CC14) has been the subject of several investigations. For instance, lowering dietary vitamin E or protein has been shown to increase the susceptibility of rats to the lethal or necrogenic effects of CC14 (1-3), but a protein-free diet was demonstrated by McLean and McLean (4,5) to have the reverse effect.Although several studies have been concerned with the effects of CC14 intoxication on in vitro liver carbohydrate metabolism (6-8), little attention has been paid to the influence of diet therein. Therefore, studies were initiated to determine how different dietary treatments, particularly those known to afford some protection against the gross effects of CC14, influence the animal's response to this toxic chemical at the cellular level. The influence of vitamin E and of protein level on some aspects of in vitro liver carbohydrate metabolism as affected by CC14 administered in vivo was investigated. Materials and Methods. Animal treatments.Weanling male albino rats of the Osborne-Mendel strain1 were maintained on a purified diet of the following composition (%) : vitamin-free casein,2 18; sucrose, 63.8; stripped lard, 9 ; cod liver oil, 1; Jones-Foster salt mixture, 4; vitamin E-free vitamin mixture ( 9 ) , 4 ; and choline chloride, 0.2. Usually, half of the rats were fed this vitamin E-deficient diet and the other half received the same diet supplemented with 1001 mg of m-alpha-tocopherol acetate/kg of diet. In an-1 Division of Nutrition colony, Food and Drug