A method is proposed for estimating the percentage of dietary protein that is degraded by microbial action in the rumen when protein supplement is added to a specified ration. The potential degradability ,p,is measured by incubating the supplement in artificial-fibre bags in the rumen and is related to incubation time, t, by the equation/) = a + 6( 1 -e~c t ). The rate constant k, measuring the passage of the supplement from the rumen to the abomasum, is obtained in a separate experiment in which the supplement is combined with a chromium marker which renders it completely indigestible. The effective percentage degradation, p, of the supplement, allowing for rate of passage, is shown to be p = a + [bc/(c + k)] (1 -e~( c +*") by time, t, after feeding. As t increases, this tends to the asymptotic value a + bc/(c + k), which therefore provides an estimate of the degradability of the protein supplement under the specified feeding conditions.The method is illustrated by results obtained with soya-bean meal fed as a supplement to a dried-grass diet for sheep. The incubation measurements showed that 89 % of the soya-bean protein disappeared within 24 h and indicated that it was all ultimately degradable with this diet.When the dried grass was given at a restricted level of feeding the allowance for time of retention in the rumen reduced the estimate of final degradability to 71% (69% within 24 h). With ad libitum feeding there was a faster rate of passage and the final degradability was estimated to be 66% (65% within 24 h). TWTT? O'DTTPTTO'WThere are essentially two methods of obtaining

S U M M A R YTwo steers totally nourished by intragastric infusion of volatile fatty acids and casein were given an abomasal infusion of a microbial protein preparation (Pruteen) at eight rates with a purine input ranging from 0 to 170 mmol/day over 11 successive 5-day periods. The urinary excretion of purine derivatives relative to the purine input was measured. Negligible amounts of xanthine plus hypoxanthine were present in the urine. The relative contributions of allantoin and uric acid to the total excretion were not affected by the rate of purine infusion. Total purine derivative excretion (uric acid and allantoin) was linearly correlated with purine input. Recovery in the urine of the infused purines was 0-77. It is suggested that utilization of exogenous purines may only occur in the intestinal mucosa and that the remaining purines may be completely converted, before entering the liver, to uric acid and allantoin, which are subsequently eliminated by the renal and extrarenal routes. The differences between cattle and sheep in excretion of purine derivatives, and the implications of these differences for the use of purine excretion values in order to estimate microbial protein supply to the ruminant, are discussed.

SUMMARYThe artificial fibre bag technique was evaluated for assessing the proportions of dietary D. M. and N which disappear in the rumen. The most important factor determining the variability in disappearance from bags incubated together was the sample size in relation to bag size. For incubation of 5 g air dry feed, a bag size of 17 ✗ 9 cm was found to be adequate. There were also variations in substrate disappearance between animals and between days of incubation. It was estimated that three sheep and the measurement of substrate disappearance twice were necessary in order to obtain acceptable repeatability.The technique was found to be satisfactory as a simple and rapid guide for measuring nutrients disappearance in the rumen such as protein degradation and carbohydrate fermentation. It has been used in subsequent work to measure the effect of features of the rumen environment, such as pH and NH3 concentration, on rate of fermentation.

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