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An attempt was made to evaluate anti-anaemic properties of probiotic Moringa oleifera leaf extract using animal model. Twenty four male rats were randomly assigned to four groups and all the animals were given Phenylhydrazine orally to induce anaemia. Enterococcus durans was selected as probiotic supplement. Group 1 was provided with 300mg/kg of M.oleifera leaf extract. Group 2 was supplemented with 0.3 ml of E.durans along with 300mg/kg of M. oleifera leaf extract. Group 3 and 4 were fed with E.durans and iron supplement (1ml/rat) respectively. Blood samples were collected on a weekly basis and various haematological and biochemical parameters were analysed. The haemoglobin level was found to be higher with 14.9±0.25 g/dl for rats fed with probioticated M.oleifera leaf extract. E.durans has proliferated in gut which was verified through faecal and gut microbiota analysis. Hence, probiotic M.oleifera leaf extract was found an effectual nutraceutical supplement to treat anaemia.

The aim of the current study was to assess a scrupulous pharmacognostic cram is to enhance the constructive information with regard to its species identification, characterization and standardization of a dynamic folklore therapeutic plant Pterolobium hexapetalum. It is one of the largest scrambling shrubs belonging to the Leguminosae family having significant medicinal properties. The aerial part of the fresh and healthy plant materials was collected from the Maruthamalai hill, Coimbatore District with a strong traditional background. The confined name of the species is “Karuindu” used as medicine in Siddha, Ayurveda and folk medicine for treating various illnesses. The external morphology of study species, the anatomy of leaf and stem, physicochemical examination of plant powder, powder with diverse chemical reagents, fluorescence analysis of therapeutic powder, ash values, mineral studies, heavy metal analysis, extractive values, phytochemical analysis, GCMS analytical studies were examined with the aim of drafting pharmacopeial principles. The studied plant contains 19 bioactive chemical constituents which confirm the healing domination. The analytical assessment of study species authenticates the presence of various bioactive constituents with a broad range of remedial properties which is used to treat manifold muddle as well it provides a comprehensive insight regarding the phytoconstituents profile which could be browbeaten for the maturity of plant-supported drugs.

Acacia caesia is a medicinal plant rich in phytochemical compounds which are used for the treatment of skin diseases, sexual problems and wound healing, stomach and tooth problems. The present study deals with the preliminary phytochemical screening of A. caesia for various bioactive compounds such as alkaloids, anthraquinones, flavonoids, glycosides, phenols, tannins, saponins, steroids and terpenoids. And also quantify the bioactive compound flavonoid in the ethanol extract of A. caesia from by High Performance liquid chromatography (HPLC) analysis. The main objective of our study is to estimate the quercetin content present in A. caesia ethanolic leaf extract by HPLC method. The estimation of quercetin content in A. caesia ethanolic leaf extract was performed using shim-pack CLC ODS (4.6 × 15 mm) column and a 350 nm detector. The mobile phase consisted of 0.1 % phosphoric acid in water and acetonitrile at 80:20 v/v. The method was validated in compliance with International Conference on Harmonization [ICH] guidelines. In conclusion, the quercetin content present in the ethanolic leaf extract of A. caesiawas found to be 0.76% w/w.

The study aimed to assess the whole bacterial population and functions of two different soils (PP and TA) through the next-generation sequencing (NGS) method in the Veerakkal forest area, Western Ghats, Tamil Nadu, India. The forest soil is slightly acidic in nature and also rich in soil organic matter. The bacterial sequence reads under soil is classified taxonomically into 14 bacterial and 2 archaeal phyla, 45 classes, 88 orders, 119 families, and 146 genera. Acidobacteria is found to be the most populated phyla accounting for 25% population in PP soil, whereas 35% of Proteobacteria is the most abundant phyla in TA soil. The PP soil has more OTUs and a higher Shannon index than the TA soil which indicates a diversity gradient of soil bacteria restricted in the study site. Using PICRUSt functional analysis, the KEGG function of level I is discovered that Metabolism has the largest amount of genomic sequences involved (PP – 56%, TA – 54%), and KEGG level II identifies a greater proportion of the community involved in Amino acid metabolism (PP and TA – 14%) followed by Carbohydrate metabolism (PP and TA – 11%), Membrane transport (PP and TA – 10%) and Energy metabolism (PP and TA – 8%), all of these are most frequent categories in the study site. The presence of carbohydrate metabolism at above 11% has confirmed the study samples for bacterial potential towards cellulolytic activity in the soil environment. Further, this study suggests that the revealed bacterial isolates have the potential in recycling agro by-products and their utilization in industrial applications.