Objective-To study whether an injury-induced inflammation might be the mechanism underlying the favorable effect of endometrial biopsy on the implantation rate in in vitro fertilization (IVF) patients.
Design-Controlled clinical study.Setting-A medical center IVF unit and a research institute.
Patient(s)-Women undergoing IVF who had previous failed treatment cycles.Intervention(s)-Endometrial samples were collected from two groups of patients on day 21 of their spontaneous menstrual cycle. The experimental, but not the control group underwent prior biopsy treatment on days 8 or/and 11 to 13 of that same cycle.Main Outcome Measure(s)-Abundance of immune cells, cytokines/chemokines level, correlation between these parameters and pregnancy outcome.Result(s)-A statistically significantly higher amount of macrophages/dendritic cells (HLA-DR + CD11c + cells) and elevated proinflammatory cytokines, tumor necrosis factor-α (TNF-α), growthregulated oncogene-α (GRO-α), interleukin-15 (IL-15), and macrophage inflammatory protein 1B (MIP-1B), were detected in day-21 endome-trial samples of the experimental group. A direct stimulatory effect of TNF-α on MIP-1B, GRO-α, and IL-15 messenger RNA (mRNA) expression was demonstrated. A positive correlation was found between the levels of macrophages/dendritic cells, MIP-1B expression, and TNF-α expression and the pregnancy outcome.
Conclusion(s)-A biopsy-induced inflammatory response may facilitate the preparation of the endometrium for implantation. Increased MIP-1B expression could possibly serve for prediction of implantation competence.
Dynamic transperineal ultrasound is a simple and accurate technique for assessment of the pelvic floor and soft-tissues in patients with evacuatory dysfunction.
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