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ABSTRACT

Background and Design: The objective of this study was to investigate the wound healing activity of different concentrations of liposomal trans-resveratrol formulations on incisional and excisional wounds in rats. Materials and Methods: The wound healing effect was tested by an excisional and incisional wound model. Wound closure was measured for 12 days. On the 12 th day of the study, maximal load, maximum stress, stress, and % of elongation values were evaluated in the incisional wound. In addition, angiogenesis, granulation tissue thickness, epidermal and dermal regeneration values, and macroscopic photographic analyses were evaluated in the excisional wound. Results: When the wound tissue surface healing rates were evaluated, similar effects were observed at the end of the 10 th and 12 th days between the 5% Res group and the commercial product containing 1% Centella asiatica extract used as the reference molecule. Histological evaluation showed that 1% Res and 5% Res groups induced significant wound healing activity compared to the control group. Furthermore, 1% Res and 5% Res groups increased wound healing rates by promoting granulation tissue, epidermal, and dermal regeneration as well as angiogenesis. Conclusion: Liposomal formulations containing 1% and 5% resveratrol were found to have positive effects on the healing process, both on excisional and incisional wound tissues.

Objective: Liposomal cancer treatment strategies are useful in removing the side effects that were the main concern in recent years. In this study, we prepared microbubble (MBs) conjugated with DOX-loaded liposomes (DOX-loaded MBs) and investigated their effectiveness in in vitro breast cancer cells in two dimensions (2D) and three dimensions (3D).Materials and Methods: With this aim, breast cancer cells with different features (4T1, MDA-MB231, MCF-7) were growth in 2D and 3D dimensions. The cytotoxic and cell death effects under different conditions, durations and doses were evaluated with WST-1, trypan-blue, colony counts. Apoptotic effects were investigated with flow cytometric Annexin-V-PI and immunohistochemical (Ki-67, caspase 3, 8, 9) methods.Results: After free DOX and LipoDOX were applied, the proliferation index of three cell lines reduced. Intrinsic and extrinsic apoptotic pathways were activated in both 2D and 3D models. However, this effect was observed at lower levels in the 3D model due to the difficulty of diffusion of DOX into the spheroids. Additionally, the suitability of the 3D model for breast cancer cells was supported by formation of ductus-like structures and spheroids. Cell deaths were not observed significantly with the DOX-loaded microbubbles due to rising of MBs to the surface and not reaching spheroids held in matrigel of 3D model. Conclusion:DOX and LipoDOX showed anti-proliferative and apoptosis-inducing effects in breast cancer cells. However, these effects indicated variability depending on the cell lines and 2D or 3D model types.

Objective: The aim of this study was to investigate the effects of naringin, a known flavonoid, on in vitro cytotoxicity, irritation and in vivo potential efficacy when topically applied to the diabetic wound. Material and Method: In vitro direct contact assay and hen's egg chorio-allantoin membrane tests were used to evaluate irritation, and cytotoxicity potential of Naringin. In vitro antimicrobial activity was also tested. Topical treatments were administered once a day on the wound. Wound lesions were photographed and statistically analyzed. After the 10th day, histopathological parameters of tissues were assessed.