Banana (Musa spp. family Musaceae) is a fourth most important fruit crop in the world. This investigation was carried out in tissue culture laboratory in Horticulture Department, Agriculture Faculty, Suez Canal University, Ismailia during the period 2013 -2015, to study the effect of medium type (solid or liquid) and cytokinin types (BAP or Kin at 0, 1, 3, 5, 7 mg/l) during multiplication stage, in a complete randomized design with two factors. The explants were collected from suckers grown around banana fruiting mother plants, cultivar Grande Naine. In liquid medium supplemented with 3.0 mg/l Benzyl amino purine (BAP) increased number of shoots (7.20). While 5 mg/l Kinetin (Kin) improved shoot length (7.40 cm) in solid medium compared with other treatments. The liquid media is preferable within mass production and commercial advantages. The BAP promotes shoot initiation and development either with solid or liquid media more than Kin.
Five plant extracts and oils namely green tea extract, turmeric extract, black seed oil, onion oil and garlic oil each at 2% were tested for evaluating their effect on yield and fruit quality of the Naomi Mango trees, during 2017-2018 seasons, these extracts were sprayed two times, one at full bloom and second at pea stage. The plant extract improve all physical and chemical characters, respectively. Turmeric extract, onion oil and garlic oil were more effective to improve tree yield, fruit shape, firmness, gave the best red color and decreased the enzyme activities. Green tea extract increased vitamin C and total phenols. Black seed oil increased fruit acidity percentage and showed the lowest ratio of TSS/ TA. Generally, foliar sprays of plant extracts and oils improved fruit quality and help in delay fruit ripening, which is important in fruit marketing.
T HE experiments were performed using (Nour, Fedela, Spinosa clementines, Kishu seedless, Balady, and Seedless mandarins) to determine the compatibility or incompatibility as related to the number of seeds per fruit and the genetic relation between them. For two consecutive seasons (2020 and 2021), the growth of the pollen tube in the styles following self-pollination has been analyzed using a fluorescence microscope to detect incompatibility properties. According to Nour and Fedela clementine cultivars are highly incompatible, while the other four cultivars are incompatible. After receiving pollen, Nour and Fedela clementines had very low pollen tubes that reached style. On the other hand, the highest rate was in the other four cultivars. The DNA of the six cultivars of mandarin and clementine investigated in this study was very similar. Balady mandarin and seedless mandarin appear to have very high similarities, while Fedela clementine and seedless mandarin appear to be very low. RAPD-PCR showed a specific molecule related to incompatibility such as (A-18) RAPD primer, at MW 361 bp. linked to the incompatibility of Nour and Fedela clementine c.vs.
This study was carried out during 2017 and 2018 seasons to study the effect of boric acid, Magnesium sulphate and some amino acids (Tryptophan, Methionine and Arginine) at 0.05%, 0.2% and 0.1% respectively, either alone or combination on solving clusters looseness, shot berries and coloration problems in Roomy Red grapevines grown under Minia region. Vegetative growth, vine nutritional status, berry setting %, yield, cluster weight as well as physical and chemical characteristics of the berries were positively affected by using amino acids, magnesium sulphate and boric acid either as a single or in combinations when compared to the check treatment. Also, shot berries % and total acidity reduced greatly with application of such treatments. Using boric acid, magnesium sulphate and amino acids influenced positively the studied characters in ascending order. Combined applications showed more effects rather than single ones in this respect. Three sprays of a mixture containing boric acid, Magnesium sulphate and some amino acids (Tryptophan, Methionine and Arginine) at 0.05%, 0.2% and 0.1%, respectively, was responsible for obtaining higher yield, lower shot berries and clusters looseness as well as promoting berries quality of Roomy Red grapevines.
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