Here are the proofs of your article.• You can submit your corrections online or by fax.• For online submission please insert your corrections in the online correction form.Always indicate the line number to which the correction refers. • Please return your proof together with the permission to publish confirmation.• For fax submission, please ensure that your corrections are clearly legible. Use a fine black pen and write the correction in the margin, not too close to the edge of the page.• Remember to note the journal title, article number, and your name when sending your response via e-mail, fax or regular mail.• Check the metadata sheet to make sure that the header information, especially author names and the corresponding affiliations are correctly shown.• Check the questions that may have arisen during copy editing and insert your answers/corrections. • Check that the text is complete and that all figures, tables and their legends are included. Also check the accuracy of special characters, equations, and electronic supplementary material if applicable. If necessary refer to the Edited manuscript.• The publication of inaccurate data such as dosages and units can have serious consequences. Please take particular care that all such details are correct. • Please do not make changes that involve only matters of style. We have generally introduced forms that follow the journal's style. Substantial changes in content, e.g., new results, corrected values, title and authorship are not allowed without the approval of the responsible editor. In such a case, please contact the Editorial Office and return his/her consent together with the proof. • If we do not receive your corrections within 48 hours, we will send you a reminder. Please noteYour article will be published Online First approximately one week after receipt of your corrected proofs. This is the official first publication citable with the DOI. Further changes are, therefore, not possible.

Resumo -Os objetivos deste trabalho foram avaliar a resposta de mudas de cedro (Cedrela fissilis Vell.) a espécies de fungos micorrízicos arbusculares (FMA) e determinar o grau de dependência micorrízica dessas mudas. O ensaio foi conduzido em casa de vegetação, em delineamento de blocos inteiramente casualizados, em esquema fatorial 5 (doses de P) x 5 (4 espécies de FMA e testemunha sem inoculação), com seis repetições. Glomus clarum foi a espécie de FMA mais eficiente em promover o crescimento e a nutrição fosfatada de mudas de cedro, principalmente quando a inoculação foi realizada em substrato com P disponível em torno de 12 mg dm -3 . A economia de P aplicado equivale a aproximadamente 63%, em relação à testemunha sem inoculação, quando foram buscados 80% do crescimento máximo. Verificou-se elevado grau de dependência micorrízica das mudas de cedro e, portanto, a inoculação de FMA é um procedimento que deve ser considerado na fase de viveiro, para garantir o sucesso do estabelecimento e crescimento em campo dessas mudas.Termos para indexação: Cedrela fissilis, endossimbiose, nutrição fosfatada, eficiência simbiótica, fósforo, inoculação. Cedar seedlings dependency and responsiveness to arbuscular mycorrhizal fungiAbstract -The objectives of this work were to evaluate cedar (Cedrela fissilis Vell.) seedlings responsiveness to arbuscular mycorrhizal fungi (AMF) species and to determine mycorrhizal dependency degree of this seedling. The experiment was carried out at greenhouse, in a complete randomized block design as a factorial set 5 (P doses) x 5 (4 AMF species + noninoculated treatment) with six replications. Glomus clarum was the most efficient AMF species in promoting the growth and P nutrition of cedar seedlings, especially when the inoculation was done in substratum containing P available levels close to 12 mg dm -3 . The saving of applied P was approximately 63%, regarding noninoculated treatment when 80% of the maximum growth was estimated. The elevated degree of mycorrhizal dependency of cedar seedlings was verified, so AMF inoculation is a procedure that should be considered in nursery stage, in order to guarantee cedar seedlings successful establishment and growth on field conditions.

-The objective of this work was to evaluate the ability of several P-solubilizing fungi to solubilize aluminum phosphate and Araxá apatite as well as the synergism between the P-solubilizing fungus, PSF 7, and arbuscular mycorrhizal fungi to promote clover growth amended with aluminum phosphate. Two experiments were carried out, the first under laboratory conditions and the second in a controlled environmental chamber. In the first experiment, PSF 7, PSF 9, PSF 21 and PSF 22 isolates plus control were incubated in liquid medium at 28ºC for eight days. On the 2 nd , 4 th and 8 th day of incubation, pH and soluble P were determined. In the second experiment, clover was sowed in plastic pots containing 300 g of sterilized substrate amended with aluminum phosphate, 3 g L -1, in presence and absence of PSF 7 isolate and arbuscular mycorrhizal fungi. A completely randomized design, in factorial outline 2x2 (presence and absence of PSF 7 and arbuscular mycorrhizal fungi) and five replicates were used. In the first experiment, higher P content was detected in the medium containing aluminum phosphate. PSF 7 is the best fungi isolate which increases aluminum solubilization with major tolerance to Al 3+ . Clover growth was stimulated by presence of PSF 7 and arbuscular mycorrhizal fungi. There is synergism between microorganisms utilized to improve plant nutrition.Index terms: Aspergillus, Trifolium pratense, Araxá apatite, aluminum phosphate, symbiosis, Glomus. Solubilização de fosfatos e sinergismo entre fungos solubilizadores de fosfato e micorrízicos arbuscularesResumo -O objetivo deste trabalho foi avaliar a capacidade de solubilização de fosfato de alumínio e apatita de Araxá por diversos isolados de fungos solubilizadores de fosfato e o sinergismo entre o fungo solubilizador de fosfato, FSF 7, e fungos micorrízicos arbusculares, na promoção do crescimento de trevo fertilizado com fosfato de alumínio. Dois experimentos foram conduzidos, o primeiro em laboratório e o segundo em câmaras de cultivo. No primeiro experimento, os isolados FSF 7, FSF 9, FSF 21 e FSF 22, mais controle foram incubados em meio líquido, sob agitação, a 28ºC, por oito dias. Avaliou-se o pH e P solúvel no segundo, quarto e oitavo dia de incubação. No segundo experimento, semeou-se trevo em copos de plástico contendo 300 g de substrato esterilizado, fertilizado com fosfato de alumínio, 3 g L -1, com e sem FSF 7 e fungos micorrízicos. O delineamento foi inteiramente casualizado, esquema fatorial 2x2 (presença e ausência de FSF 7 e fungos micorrízicos), com cinco repetições. No primeiro experimento, foram encontradas maiores quantidades de P solúvel no meio contendo fosfato de alumínio. O FSF 7 aumentou a solubilização desse fosfato. O crescimento do trevo foi favorecido pela presença do FSF 7 e fungos micorrízicos arbusculares, o que evidencia sinergismo entre esses microrganismos.Termos para indexação: Aspergillus, Trifolium pratense, apatita de Araxá, fosfato de alumínio, simbiose, Glomus.

Resumo -O objetivo deste trabalho foi avaliar a capacidade e a eficiência de solubilização de CaHPO 4 , AlPO 4 e apatita de Araxá em meio sólido, e de AlPO 4 e apatita de Araxá em meio líquido, por fungos (Aspergillus) e bactérias (Enterobacteriaceae) do solo. Em meio sólido, todos solubilizaram CaHPO 4 , nenhum solubilizou apatita de Araxá e apenas o isolado de fungo FSF 7 solubilizou AlPO 4 . Em meio líquido, todos solubilizaram AlPO 4 e apatita de Araxá. A seleção de solubilizadores deve ser feita com a quantificação do potencial de solubilização em meio líquido.Termos para indexação: Aspergillus, enterobactérias, fósforo, meio de cultivo, apatita. Phosphate solubilization in solid and liquid media by soil bacteria and fungiAbstract -The objective of this work was to evaluate the ability and efficiency of solubilization of CaHPO 4 , AlPO 4 and Araxá apatite in solid medium, and AlPO 4 and Araxá apatite in liquid medium. Soil P-solubilizing fungi (Aspergillus) and P-solubilizing bacteria (Enterobacteriaceae) were tested. In solid medium, all isolates solubilized CaHPO 4 , not any isolate solubilized Araxá apatite, and one fungus isolate (PSF 7) solubilized AlPO 4 . In liquid medium, all isolates solubilized AlPO 4 and Araxá apatite. Screening of P-solubilizing microorganisms must be done by quantifying their potential of phosphate solubilization in liquid growth medium.

No abstract