While there has been progress in our understanding of the origin and history of agriculture in sub-Saharan Africa, a unified perspective is still lacking on where and how major crops were domesticated in the region. Here, we investigated the domestication of African yam (Dioscorea rotundata), a key crop in early African agriculture. Using whole-genome resequencing and statistical models, we show that cultivated yam was domesticated from a forest species. We infer that the expansion of African yam agriculture started in the Niger River basin. This result, alongside with the origins of African rice and pearl millet, supports the hypothesis that the vicinity of the Niger River was a major cradle of African agriculture.

A cassava seedbed preparation field study was established at Fumesua in the forest and Ejura in the forest-savanna transition agroecologies of Ghana in 2004/2005. The experimental design was split plot with three seed bed preparation methods as the main plots and three nitrogen rates as sub plots, with basal application of 45-90 kg ha -1 P 2 0 5 -K 2 0 on the fertilized plots. A similar study was conducted on yam seedbed preparation in 2003/2004, with a 2 3 factorial design. Cassava and yams on ridges resulted in highest root and tuber yields, on both Lixisols in the coastal and forest-savanna transition and Acrisols in the forest agro-ecologies. However, yam tuber yield on mounds was not statistically different from yields on ridges. Number of roots per plant was identified as a major contributory factor to the yield increase of cassava on ridges. Seed bed preparation method was, however, not an important determinant of cassava root yield at high rates of fertilizer application of 90-45-90 kg ha -1 N-P 2 0 5 -K 2 0. Planting on mounds resulted in slender, cylindrically shaped cassava roots and yam tubers, while ridging produced oblong shaped roots and tubers. Weeding and fertilizer application were easier on manual ridging than on manual mounds. The study points to ridging as a potential option to mounding for cassava and yam production, with the feasibility of mechanization of ridges to reduce drudgery associated with roots and tuber crop production in the West African sub-region.

Fourteen varieties of indica rice (Oryza saliva L.) were examined for their capacity for plant regeneration from protoplasts using the nurse culture methods developed for japonica rice. Calli induced from germinating seeds were grouped into two types: type I, white and compact; type II, yellow and friable. In four varieties producing type II callus, colony formation (2%-4.5%) and plant regeneration (2%-35%) were observed. The inability to develop suspension cultures was a major obstacle in regenerating plants from protoplasts of the remaining rice varieties studied.

The yam species, Dioscorea alata has an advantage for sustainable cultivation due to its comparatively good agronomic characteristics. Breeders are therefore keen to improve the food quality of the species. Nevertheless, published data on molecular classification and genetic diversity of this crop are scanty. This research therefore investigated genetic variability and relationships among some collected Ghanaian D. alata accessions (35) together with 14 introductions from IITA in Nigeria. The true genetic identity and population structure of the accessions were determined using 14 Simple Sequence Repeats primer pairs available for yams. Dimensional scatter diagram of the principal coordinates showed a wide dispersion among the accessions. Cluster analysis using unweighted neighbour-joining method clearly separated the 49 accessions into five main groupings. From the allele frequency analysis, the size of amplified alleles ranged from 100 to 510 bp. The mean polymorphic information content (PIC) values for all markers used was 0.91 and ranged between 0.86 and 0.94 in loci YM13 and YM30 respectively. Gene diversity was high and the average observed heterozygosity was 0.77. Gene diversity was high ranging from 0.87 in YM13 to 0.94 in YM30 with a mean of 0.92. Generally, the allele frequency of all the primers was below 0.95 indicating that they were all polymorphic in character. The findings of this study confirm that SSR molecular markers are able to identify closely related materials within species.

Guided by the theory of reasoned action, this study found support for the hypothesized inverse relationship between work-related factors and employee turnover intention on the one hand, and turnover intention and perceptions of accountable absence legitimacy on the other hand. Specifically, the higher employees' turnover intent, the lower their perceptions about the accountability of their absence behavior and vice versa. The findings highlight the need to consider turnover intention as a salient construct that plays a dual role, first as a consequence variable to job satisfaction, affective and normative commitments, and next as an antecedent to accountable absence legitimacy in the workplace. The article concludes with a discussion on the significance of lessening employee turnover intent as a means of mitigating the perceptions of absence legitimacy in the workplace.Growing Science Ltd. All rights reserved. 5