ABSTRAKSalah satu penyakit akibat kerja yang dapat muncul sewaktu-waktu adalah Musculoskeletal Disorders (MSDs). Keluhan Musculoskeletal Disorders (MSDs) adalah keluhan pada bagian otot skeletal yang dirasakan oleh seseorang mulai dari keluhan yang sangat ringan sampai sangat sakit. Musculoskeletal Disorders (MSDs) dapat dipengaruhi beberapa faktor yang terdiri dari umur, masa kerja, dan sikap kerja. Penelitian ini bertujuan untuk mengetahui hubungan antara umur dan masa kerja keluhan Musculoskeletal Disorders (MSDs) pada pekerja Laundry di Jalan Prof. Dr. Soepomo Janturan Yogyakarta. Penelitian ini merupakan penelitian Cross Sectional. Responden merupakan pekerja laundry di Jalan Prof. Dr. Soepomo Janturan Yogyakarta yang berjumlah 35 responden. Pengumpulan data dilakukan dengan menggunakan kuesioner Nordic Body Map. Analisis bivariat dengan uji statistik Chi Square dan uji alternatifnya yaitu uji Fisher. Responden yang mengalami keluhan Musculoskeletal Disorders (MSDs) lebih banyak yaitu 22 responden (62,9%). Responden yang berumur >30 tahun lebih banyak yaitu 20 responden (57,1%). Responden yang memiliki masa kerja <5 tahun lebih banyak yaitu 30 responden (85,8%). Ada hubungan antara umur dengan keluhan MSDs dengan nilai p= 0,005 < α (0,05) dan tidak ada hubungan antara masa kerja dengan keluhan MSDs dengan nilai p= 0,630 > α (0,05). Ada hubungan antara umur dengan keluhan MSDs namun tidak ada hubungan antara masa kerja dengan keluhan MSDs. Kata kunci: Musculoskeletal Disorders (MSDs), umur, masa kerja Correlation Between Age and Working Periods with Musculoskeletal Disorders (MSDs) in Laundry Workers ABSTRACT One of occupational diseases that can appear any time is Musculoskeletal Disorders (MSDs).Complaints about Musculoskeletal Disorders (MSDs) are the complaints on the part of the musculoskeletal felt by a person in such a deep ranging from complaints of mild to hard. Muscuoskeletal Disorders (MSDs) can be caused by any factors such as age, length of work and work positions. This research aimed to determine the relationship between age and length of work with Musculoskeletal Disorders (MSDs) complaint towards laundry worker at Prof. Dr. Soepomo Street, Janturan, Yogyakarta, Indonesia. This study employed Cross Sectional design. The rspondents of the study were laundry worker at Prof. Dr. Soepomo street Janturan Yogyakarta, amounted 35 people. The data collected using Nordic Body Map questionnaire. The data analyzed statistically by Chi Square test and the alternative test that was Fisher. There were 62.99% respondents suffered MSDs, while 57.1% rspondents were more than 30 years old. Respondents who had length of work <5 years were 85.5%. There was a relationship between age and MSDs complaints p= 0.005 < α (0.05) but there was no relationship between length of work with MSDs complaints with p= 0.630 > α (0.05). There was a relationship between age with MSDs complaints but there was no relationship between length of work with MSDs complaints.