Background: Brucellosis is an endemic enzootic disease that can involve many organs and tissues. Brucella epididymo-orchitis is a focal complication of the human brucellosis. This study present epidemiologic, clinical and laboratory features of patients suffering from Brucella induced epididymo-orchitis, in comparison with cases of nonspecific epididymo-orchitis.Methods: this analytical and cross sectional study was performed in valiasr hospital in arak from 2007 to 2011,40 case of Brucella epididymo-orchitis were compare with 40 of bacterial(non specific) epididymo-orchitis and then statistics were analyzed by spss-ver.16 and by Student T test & Mann-Whitney U test & chisquare test.Results: In this study there was no significant difference between the age (P value = 0.8), fever (P value = 0.17), history of urologic manipulation (P value = 0.23), signs of STD (P value = 0.6), increased ESR (P value = 0.28) and positive CRP (P value = 0.45), of two groups, but there was a significant difference between the presence of arthritis & arthralgias (P value = 0.02), leukocytosis (P value <0.05), pyuria (P value = 0.002) symptoms of dysury -frequency (P value = 0.004), presence of testicular abscess, (P value = 0.021) in sweating (P value <0.05) and location (P value <0.05), in the two groups.Conclusion: This study showed in the existence of epididymo-orchitis without symptoms like: dysuria and frequency,leukocytosis and abnormal urine analysis is suggestive of brucella epididymoorchitis. The physicians In endemic areas can faster to use of these findings in the diagnosis and treatment of Brucella epididymo-orchitis.