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has attracted a great interest in the past decades due to its extraordinary resistance to ionizing radiation and highly efficient DNA repair system. Recent studies indicated that is a putative pleiotropic gene in and plays an important role in radioresistance and antioxidation, but its underlying mechanisms are poorly elucidated. In this study, mutation was generated to investigate resistance to desiccation and oxidative stress. The result showed that the survival of mutant under desiccation was markedly retarded compared to the wild strain from day 7-28. Furthermore, knockout of increases the intercellular accumulation of ROS and the sensibility to HO stress in the bacterial growth inhibition assay. The absorbance spectrum experiment for detecting the carotenoid showed that deinoxanthin, a carotenoid that peculiarly exists in Deinococcus, was reduced in the mutant in the mutant. Quantitative real time PCR showed decreased expression of three genes viz. (DR0861, 50%), (DR0862, 40%) and (DR0093, 50%), which are involved in deinoxanthin synthesis, and of Dps (DNA protection during starving) gene (DRB0092) relevant to ion combining and DNA protection in cells. Our results suggest that may affect antioxidative ability of by regulating the synthesis of deinoxanthin and the concentration of metal ions. This may provide new clues for the treatment of antioxidants.

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