Background Diabetes is a common disease that causes gingival and periodontal problems. Stem cells isolated from dental sources are an emerging area of research with a potential to facilitate regenerative medicine. The stem cells retain the property of self-renewal and the ones isolated from dental sources are mainly multipotent mesenchymal stem cells that have the ability to self-renew as well as differentiation towards multiple lineages. Objectives We aimed to isolate and characterize gingival mesenchymal stem cells by pluripotency markers and investigated the effect of oxidative stress on growth kinetics and apoptotic gene expression of gingival cells exposed to glucose mediated oxidative stress. Methods In this study, we isolated gingival mesenchymal stem cells from gingiva. This was followed by morphologic analysis using inverted phase contrast microscopy and molecular profiling of these cells for the mRNA expression of specific genes. The isolated cells were cultured till passage 3 and then exposed to oxidative stress (high glucose concentration). We measured the apoptotic gene expression and compared their growth kinetics. Results The results showed that oxidative stress produced by glucose reduced growth kinetics and increased apoptotic gene expression in gingival mesenchymal stem cells. According to the genetic results, glucose activated TNF family to initiate apoptosis. Conclusion In conclusion, the present study demonstrated that high glucose obliterated cellular proliferation testified by evaluating growth kinetics and induced apoptotic gene expression in gingival mesenchymal stem cells. This initiated extrinsic apoptotic pathway mediated by TNF family. Therefore, in diabetes oral health condition is compromised and periodontal disease is common.

Objectives The perceptions maintained by the teaching profession have a plausible influence on the effectiveness of online teaching practices, but they have not been well comprehended in many institutions in Pakistan. This study is the first to demonstrate how different academic disciplines address the challenges and opportunities regarding online teaching during coronavirus disease 2019 (COVID-19) and whether there exists a strong correlation between respondents' attitudes and their field of discipline. Materials and Methods A multi-centric cross-sectional survey was conducted online, and data gathered from the online survey were analyzed using SPSS version 16, demographic and categorical data were analyzed in frequencies and percentages. A relationship between the attitudes of teachers with their areas of discipline and a difference in response between genders and age groups were analyzed using a chi-square test. Results The findings of our study provide valuable understandings that most of the higher education faculty felt positively (64.1%) regarding the online mode of teaching. However, their perceived attitudes have a significant influence on the nature of their academic discipline. Conclusion These findings provide relevant suggestions to the core aspects of the use of the e-learning system during the COVID-19 pandemic to foster positive teaching experiences. The administrations and institutions can use it to identify the e-learning constraints within multiple fields of profession and help instructors generate quality materials that will reshape the framework of online education and preserve the practice of blended teaching even after the pandemic.

Objectives: To Determine the efficiency of wax removal from denture baseresin using hot water at a range of temperatures with added detergentand to Study the effectsof mechanically modified surfaces of denture base resin on the efficiency of dewaxing usingthe above techniques. Settings: Eastman Dental Institute University Of London UK. Period:Jan 2007 to June 2007. Material and Methods: In this study cast acrylic rods 13 mm longand 6 mm in diameter were used. Three different surfaces were prepared for each rod,described as Polished, Abraded & Grooved. Nine experimental groups, each consisting of tenspecimens were mechanically modified were contaminated with modeling wax, and then thosein each group were subjected to agitation in hot water with detergent at different range oftemperatures. The surfaces were then examined for traces of wax using Raman spectroscopy.Results: The spectrum for each specimen demonstrated peaks at different wavelengths andvarying intensities, the spectra demonstrated traces of wax on abraded surfaces showedleast wax when dewaxed at 100°C as compared abraded surfaces dewaxed at 65°C and 85°C temperatures respectively. Conclusion: The rough surfaces (abraded or grooved) showedmore wax retention as compared to polished surfaces. These surfaces are usually prepared bythe dental technician in a belief that modifying the ridge-lap area of the teeth will increase thebond strength between the teeth and the denture base. Residual wax can still remain on thetooth surface even after dewaxing it at 100˚C with detergent added to the water.

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To Determine the efficiency of wax removal from denture base resin using hot water at a range of temperatures with added detergentand to Study the effects of mechanically modified surfaces of denture base resin on the efficiency of dewaxing using the above techniques.