The study was conducted to assess the influence of BAP and NAA on in vitro plantlet regeneration of local and exotic banana cultivars. Shoot tip of the cultivars Grand Naine, Amritasagar and Sabri were used as explants for establishment of culture. Different concentrations of BAP (1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 mg/L) were used in MS medium to study their effects on shoot formation. Explants of all the cultivars produced both single and multiple shoots but rate of shoot regeneration varied among different cultivars and BAP concentrations. The highest shoot regeneration was found in all the three cultivars at 5.0 mg/L of BAP. Among the three banana cultivars Grand Naine produced the highest shoot. Shoot proliferation rate of Grand Naine, Amritasagar and Sabri was 3.50, 3.25 and 1.75 (per explant) respectively was obtained after 30 days of culture. MS medium supplemented with different concentrations of NAA (0.5, 1.0, 2.0, 4.0 and 6.0 mg/L) were used for in vitro root formation from proliferated shoots. The cultivars Grand Naine showed the best root forming performance in all concentrations of NAA. Best results of root formation were observed on MS medium supplemented with 4.0 mg/L NAA. All the plantlets regenerated in vitro from shoot tip explants of three banana cultivars survived in the field condition but Grand Naine showed the superior performance.

Breast cancer affects one in eight women in Bangladesh and is the most common cancer among women in South Asia next to skin cancer. NUDT5 are nucleotide-metabolizing enzymes (NUDIX hydrolases) linked with the ADP ribose and 8-oxo-guanine metabolism. It is known to be associated with the hormone dependent gene regulation and proliferation in breast cancer cells. It blocks progestin-dependent, PARderived nuclear ATP synthesis and subsequent chromatin remodeling, gene regulation and proliferation in this context. We describe the structure based binding features of a lead compound (7-[[5-(3, 4-dichlorophenyl)-1,3,4-oxadiazol-2-yl]methyl]-1,3-dimethyl-8piperazin-1ylpurine-2,6-dione-C20H20Cl2N8O3) with NUDT5 for further in vitro and in vivo validation. It is a promising inhibitor for blocking NUDT5 activity. Thus, structure based virtual screening is used to identify a potential therapeutic inhibitor for NUDT5.

Broiler meat is one of the most important protein sources for Bangladeshi people. Food-borne diseases associated with the consumption of poultry meat and its processed products are of public health concern worldwide. An investigation was conducted to assess the bacteriological quality of poultry meat from some poultry farms and its health impact on consumer of Nalitabari paurosova, Sherpur district, Bangladesh. Total 15 samples were randomly selected and collected from different poultry farms on the basis of farms level and size. Bacteriological quality of the samples was assessed by following the standard microbiological methods. The health impact was evaluated with the help of semi-structured based questionnaire of 400 peoples. The average value of TVC and TCC were found as 4.3 × 10 6 CFU/g and 3.6 × 10 4 CFU/g respectively. In this study, the prevalence of fecal coliform was recorded as 33% and the presence of E. coli in 53% samples. The mean value of Salmonella spp. of meat samples was 4.6 × 10 3 CFU/g. No Shigella spp., Vibrio spp. and fungal species were detected in any sample. Some selected isolates were tested for their sensitivity against some commercially available common antibiotics used in Bangladesh. E. coli was 80% resistance to Ampicillin and 90% sensitive to Ciprofloxacin whereas Salmonella spp. showed 100% resistance to Ampicillin and 80% sensitivity to Ciprofloxacin. The antibacterial activity of renowned medicinal plant Azadirachta indica was also evaluated against some multidrug resistance bacteria. The inhibitory zone of both 30% methanolic and ethanolic extracts of Azadirachta indica was 12 mm and 12.3 mm, where 40% methanolic and ethanolic extracts were 14 mm and 16.3 mm against How to cite this paper: Faruque582Advances in Microbiology E. coli. The 40% ethanolic extract showed the better activity between them.The plant extract has no activity against Salmonella spp.. Awareness and health impact of broiler meat was determined among the people of different sectors on the basis of educational qualification, socio-economic condition, income source, broiler meat intake pattern, BMI range and food related diseases they have suffered. The peoples who eat broiler meat are much more prone to complicated diseases than the peoples who never eat it. So broiler meat intake pattern must be changed for better health. The widespread occurrence of Salmonella spp. and E. coli in poultry meat also reinforces the need for effective control measures.

Water is one of the most vital elements of ecosystem and human being, but unfortunately nowadays, the pollution of surface and drinking water is an alarming problem. The present work deals with the assessment of physicochemical and bacteriological profile of several pond, jar and tube-well water samples to ensure its suitability for using and drinking. Total 30 samples were randomly selected and collected from Nakla Paurosova of Sherpur district by following the standard procedure. Bacteriological analysis was carried out by following the standard bacteriological methods. Most of the surface water sampling points were polluted by dumping of waste, cattle wash and were not suitable for drinking or other domestic purposes. Among three heavy metals, only Iron was detected in six tube-well water samples, one was also positive to arsenic, rest of the water bodies were negative to all of these metals.

Genetic variations of seventeen rice germplasms were studied using two SSR markers (RM122 and RM390) linked to the rice bacterial leaf blight (BLB) resistance genes.The average number of allele per locus was 10, with a range of 7 (RM122) to as many as 13 (RM390). The highest level of gene diversity value (0.9135) was observed in loci RM390 and the lowest level of gene diversity value (0.7059) was observed in loci RM122 with a mean diversity of 0.8097. The PIC values ranged from a low of 0.6715 (RM122) to a high of 0.9069 (RM390) and averaged of 0.7892. A dendrogram based on genetic distance using UPGMA, indicates segregation of the 17 rice varieties in six major clusters, BR10 and Binadhan 5, which were single variety formed cluster-1 and cluster-2 respectively.