Screening of Rice Varieties for Bacterial Leaf Blight Resistance by Using SSR Markers

Khan, Tasleem-uz-Zaman; Evamoni, Fatematuz Zuhura; Rubel, Mehede Hassan; Nasiruddin, KM; Rahman, MM

doi:10.18801/jbar.030115.30

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…Marker assisted selection Parental survey was carried out to identi ed markers polymorphic to blast resistance genes (RM6836, RM8225), bacterial leaf blight (Xa13Prom, pTA248, RM164 ) and drought tolerance (RM520, RM511, RM1261), these markers are also linked to the genes/QTLs of interest and were similarly used on the same traits by Ashkani et al (2011); Pinta et al (2013); Miah et al (2016); Khan et al (2015); Shamsudin et al (2016). In F 1 generation, crosses between Putra-1 (blast resistance) and MR219-PL-137 drought tolerance cultivars and between Putra-1 and IRBB60 (bacterial leaf blight) was carried out, true F 1 were those plants with heterozygous ampli cation.…”

Section: Resultsmentioning

confidence: 99%

“…Linked markers associated with the genes of interest were selected from published article for foreground selection which included; Blast resistance (Ashkani et al (2011), Pinta et al (2013), Miah et al (2016), drought tolerance (Shamsudin et al 2016). Some of the primers were mapped by Miah et al (2016), for blast resistance, (Miah et al 2016) and (Shamsudin et al 2016) for drought tolerance, and bacterial leaf blight (Pinta et al 2013;He et al 2006;Khan et al 2015;Pradhan et al 2015)as indicated in Table 1.…”

Section: Molecular Markersmentioning

confidence: 99%

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Pyramiding approach for development of blast and bacterial leaf blight resistant, and drought tolerant rice variety through marker-assisted selection

Akos¹,

Rafii²,

Ismail³

et al. 2020

Preprint

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Rice ( Oryza sativa L) is an important cereal crop and staple food crop of the world, whose yield could be influenced by biotic and abiotic conditions, resulting in 1-100% yield losses depending on the incidences of diseases or severity of stress condition. The research was basically to identify the polymorphic markers at the target region (parental survey) and screened for selection of improved lines with multiple traits of resistance/tolerance to two or three of blast, bacterial leaf blight and drought tolerance. Three varieties were used; two high yielding varieties of Putra-1 and MR219-PL-137 resistant to blast and tolerant to drought respectively, and an IRBB60 bacterial leaf blight resistance variety. They were crossed to a common recipient parent (Putra-1) to produce two categories of F 1 hybrid as baseline for development of improved lines in three crossing methods which produced five populations (PB, PD, PBD, PDB, DPB) through marker-assisted pedigree selection. The populations were screened by genotyping using SSR markers to select lines polymorphic to parental cultivars and carrying the linked markers/QTLs for diseases resistance and drought tolerance: Blast resistance (RM6836 and RM8225), IRBB60 (RM224, RG136, RM122, RM21), MR219-PL-137 drought tolerance QTLs (RM236, RM520, RM511, RM1261). The results of gel electrophoresis agreed with the phenotyping results of the improved lines F 4 , F 3 (2), F 3 for single, double and three-way (and reciprocal) crosses respectively and also susceptible parent cultivars.

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Section: Resultsmentioning

confidence: 99%

Section: Molecular Markersmentioning

confidence: 99%

Pyramiding approach for development of blast and bacterial leaf blight resistant, and drought tolerant rice variety through marker-assisted selection

Akos¹,

Rafii²,

Ismail³

et al. 2020

Preprint

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“…Gen tersebut untuk menanggulangi stress biotic, diantaranya penyakit HDB dan blas serta hama wereng cokelat. Penanda molekuler dikembangkan untuk ketahanan tanaman yang lebih tahan lama pada padi modern (Khan et al 2015).…”

Section: Evaluasi Ketahanan Genotipe Padi Terhadap Hawar Daun Bakteriunclassified

Ketahanan Varietas Unggul Baru dan Galur Isogenik Padi terhadap Penyakit Hawar Daun Bakteri pada Kondisi Lapangan

Yuliani¹,

Suprihanto²,

Sudir³

2021

j. penelit. pertan. tanam. pangan

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Bacterial leaf blight (BLB) is a major disease of rice in Indonesia. Cilacap District of Central Java Province and Kuningan District of West Java Province are two endemic areas of BLB. The field trial was carried out in Cilacap and Kuningan in planting season of 2017 to evaluate resistance to BLB of 20 rice genotypes including 10 new improved varieties, one differential variety, nine isogenic lines. The trial was arranged in a completed block design with three replications. Data collected were: 1) Incidences and severities of BLB; 2) Resistances of the rice genotypes to BLB; and 3) Pathotypes of Xanthomonas oryzae pv. oryzae (Xoo). The results showed the incidence of BLB disease in Cilacap of 0-100% and disease severity of 0 to 85.18%. In Kuningan, the disease incidence was 0-100% and the disease severity from 0 to 79.26%. The varieties of Inpari-32, Java-14, and the lines of IRBB-5, IRBB-7, IRBB-21, and IRBB-57 were resistant to BLB in Cilacap and Kuningan. Those varieties/lines were expected to be used as a source of resistance to BLB in Cilacap and Kuningan. Xoo pathotype test showed that 14 Xoo isolates from Cilacap classified as pathotype VIII, while nine Xoo isolates from Kuningan were classified as pathotype III. The planting of resistant varieties based on the existence of the dominant of Xoo pathotypes which specific in the field can reduce the incidence and the severity of BLB disease effectively and efficiently, while the assembly of BLB resistant varieties can use the resistant genes of xa5, Xa7, and Xa21 as they were still effective to control the dominant pathotypes of BLB in Indonesia.

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“…Penggunaan marka SSR untuk mengkaji keragaman genetik cabai dari beragam spesies telah dilaporkan oleh peneliti sebelumnya, di antaranya Dhaliwal et al (2014), Sharmin et al (2018), dan Terryana et al (2018). Sementara, Khan et al (2015) menggunakan marka SSR untuk menyeleksi ketahanan varietas padi terhadap penyakit hawar daun bakteri. Tasma et al (2018) menggunakan marka SSR untuk menganalisis diversitas genetik aksesi kedelai Indonesia dan introduksi dari Amerika Serikat pada program pemuliaan tanaman kedelai produktivitas tinggi dengan karakter juvenil panjang (long juvenile character).…”

Section: Pendahuluanunclassified

Genetic Diversity Analysis of 41 Chili Pepper Genotypes [Capsicum annuum L.] Based on SSR Markers

et al. 2019

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<p>Chili (Capsicum annuum L.) is an important horticultural crop which possesses high genetic diversity. Their genetic diversity needs to be assessed through molecular marker-based analysis to facilitate chili breeding scheme. The objective of the study was to analyze the genetic diversity of 41 chili genotypes using Simple Sequence Repeat (SSR) markers. Eleven SSR markers were used to amplify all genotypes through PCR technique, visualized on Polyacrylamide Gel Electrophoresis (PAGE). The data were analyzed using NTSYSpc version 2.1 and PowerMarker version 3.25 softwares. Results showed that the average of<br />SSR alleles was 7.9 with a range of 4-11 alleles per marker. All SSR markers showed PIC value >0.5, indicating their suitable use for chili genetic studies. Cluster analysis of 41 genotypes generated three main groups according to coefficient similarity of 77.4%. The genetic clusters appeared as reflection of the genetic background of each genotype. In addition, the SSR markers used can identify potential parent as indicated by the genetic distance between genotypes. The highest genetic diversity (0.698) was demonstrated by combinations of Yuni × Ayesha, Yuni × Ayesha 2, and Yuni × Namira. The estimated genetic diversity produced by SSR markers in this study should be useful as guidance for chili breeders to select suitable parents for new cultivar development for high productivity and resistance to diseases.</p>

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Screening of Rice Varieties for Bacterial Leaf Blight Resistance by Using SSR Markers

Cited by 4 publications

References 19 publications

Pyramiding approach for development of blast and bacterial leaf blight resistant, and drought tolerant rice variety through marker-assisted selection

Pyramiding approach for development of blast and bacterial leaf blight resistant, and drought tolerant rice variety through marker-assisted selection

Ketahanan Varietas Unggul Baru dan Galur Isogenik Padi terhadap Penyakit Hawar Daun Bakteri pada Kondisi Lapangan

Genetic Diversity Analysis of 41 Chili Pepper Genotypes [Capsicum annuum L.] Based on SSR Markers

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