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Objectives:The main propose of this investigation is to evaluate the value of ultrasound irradiation technique versus boiling water bath in the reconstitution cold MIBI (Sestamibi) kits by technetium 99m. Material and Methods: Twenty Sestamibi cold kits from various batches have been chosen. The vials were randomly divided into two equal groups. The first group was reconstituted by boiling water bath as standard method and the other group reconstituted by new developed technique. The stability of radio-complex samples were examined by thin layer chromatography (ITLC) and Radio high pressure liquid chromatography (Radio-HPLC) up to 24 hours post reconstitution. The partition coefficient and protein bonding of radiotracer samples were analyzed. Results: The significant differences have not been observed in the yields and stability of radiotracer samples which were prepared by either two methods in ITLC and Radio-HPLC investigations. The partition coefficient and protein bonding assays demonstrated that radiotracer samples which were prepared by boiling water bath were a little more lipophilic than the radio-complex samples which were reconstituted by new developed technique. Conclusion: Green chemistry is convenient and efficient modality to reconstitute the cold Sestamibi kit by technetium 99m. It can be established as an alternative method to prepared 99m Tc-Sestamibi in the clinical practice.

The ultrasound irradiation technique is convenient and sufficient method to prepare (99m)Tc-sestamibi. It can be recommended as an alternative method to reconstitute sestamibi kits particularly in emergency situations to reduce potentially medical risk by avoiding any delay in acute therapy for myocardial infarction.

Central glutamate, melanocortin and corticotropin systems have mediatory role on several physiologic functions in the brain, but their interactions on appetite regulation are not fully elicited. So, the aim of the current study was to determine interaction of the glutamate with melanocortin and corticotropin systems on food intake in 3-h food-deprived (FD 3 ) neonatal meat-type chicken. In experiment 1, chicken intracerebroventricular (ICV) injected (A) phosphate-buffered saline (PBS), (B) glutamate (75 nmol), (C) glutamate (150 nmol) and (D) glutamate (300 nmol). In experiment 2, (A) PBS, (B) astressin-B (CRF 1 /CRF 2 receptors antagonist, 30 µg), (C) glutamate (300 nmol) and (D) astressin-B+glutamate were ICV injected. Experiments 3-5 were similar to experiment 2, except birds were injected with astressin2-B (CRF 2 receptor antagonist, 30 µg), SHU9119 (MC 3 /MC 4 receptor antagonist, 0.5 nmol) and MCL0020 (MC 4 receptor antagonist, 0.5 nmol) instead of the astressin-B. In experiment 6, the injections were (A) PBS, (B) MTII (MC 3 /MC 4 receptor agonist, 2.5ng), (C) glutamate (75nmol) and (D) MTII+glutamate. Then, cumulative feed intake was recorded at 30, 60 and 120 minutes after injection. According to the results, dose dependent hypophagia observed by ICV injection of the glutamate (75, 150 and 300nmol) compared to control group in neonatal broiler chicken (p<0.05). Co-injection of the astressin-B+glutamate and astressin2-B+glutamate decreased glutamateinduced hypophagia in neonatal broiler chicken (p<0.05). Coinjection of the glutamate+MC 3 /MC 4 receptors antagonist decreased hypophagic effect of the glutamate (p<0.05). These results suggested hypophagic effect of the glutamate mediates via CRF 1 /CRF 2 and MC 3 / MC 4 receptors in chickens.