Lendir bekicot (Achatina fulica Ferr) mengandung protein achasin, suatu senyawa dengan aktivitas antibakteri yang mendukung proses penyembuhan luka. Selain itu, peptida mytimacin-AF pada lender bekicot diketahui menghambat pertumbuhan Staphylococcus aureus ATCC 25923. Lendir bekicot kurang praktis jika digunakan secara langsung sehingga perlu dikembangkan menjadi bentuk sediaan gel. Penelitian ini bertujuan untuk mengetahui proporsi optimum campuran carbopol 940 dengan gliserin dalam formula gel dan mengevaluasi aktivitas antibakteri formula optimum terhadap S. aureus ATCC 25923. Gel lendir bekicot dibuat tiga formula dengan komposisi carbopol 940 dan gliserin masing-masing sebesar 1; 1,5; dan 2%, serta 15; 14,5; dan 14%. Semua formula diuji sifat fisiknya selama empat minggu, meliputi organoleptis, homogenitas, viskositas, daya sebar, daya lekat, dan pergeseran viskositas. Hasil uji dioptimasi dengan metode simplex lattice design menggunakan program Design Expert 8.0.6.1. Formula optimum yang diperoleh dievaluasi sifat fisiknya selama empat minggu, sedangkan aktivitas antibakterinya diuji dengan menggunakan metode difusi sumuran. Hasil penelitian menunjukkan formula optimum gel lendir bekicot dengan proporsi campuran carbopol 940 dan gliserin masing-masing 1,123 dan 14,877%. Formula optimum memiliki aktivitas antibakteri terhadap S. aureus dengan diameter zona hambat sebesar 1,73 cm.
In order to screen natural antioxidant, the research about antioxidant of some Euphobiaceae herbs, have been conducted. The air-dried herbs of Euphorbia heterophylla L, Phyllanthus acidus (L.) Skeels, and Phyllanthus buxifolius Muell Arg were extracted with metanol. The obtained extract was concentrated and then suspended to produce n-hexane, ethyl acetat and aqueous fractions. Free radical scavenger activity against DPPH (1,1-diphenyl-2-pycrylhydrazyl) measured by spectrophotometric method and the IC50 value was determined. The compounds of active fraction had been identified by TLC method. All of the herbs showed activity as DPPH scavenger. Among these herbs, Euphorbia heterophylla L. and Phyllanthus buxifolius Muell, Arg. exhibited a strong free radical scavenging of ethyl acetat fraction with IC50 value 5,88 µg/ml and 4,64 µg/ml. The result of TLC by mobile phase n-buthanol-acetic acid-water (4:1:5) and acetic acid 15% showed flavonoid compound.
Latar Belakang: Herba ceplukan (Physalis angulata L.) merupakan salah satu dari tanaman obat yang mengadung beberapa senyawa aktif yang diduga bersifat antibakteri. Kehamilan dengan ancaman persalinan preterm dikarenakan pertumbuhan kuman Staphylococcus epidermidis. Tujuan: dari penelitian ini adalah mengetahui aktivitas antibakteri ekstrak etanol, fraksi n-heksan, etil asetat, dan air herba ceplukan terhadap bakteri Staphylococcus epidermidis. Metode: Penelitian eksperimental dengan melakukan penyarian herba ceplukan dengan metode maserasi menggunakan pelarut etanol 70%, kemudian difraksinasi dengan pelarut n-heksan, etil asetat, dan air. Hasil ekstrak dan fraksinasi diuji aktivitas antibakteri menggunakan metode difusi dengan konsentrasi 20%; 10% dan 5%. Hasil fraksi teraktif dilanjutkan uji makrodilusi. Identifikasi kandungan kimia herba ceplukan dikerjakan dengan menggunakan uji tabung dan Kromatorafi Lapis Tipis (KLT). Hasil: Penelitian menghasilkan rendemen ekstrak, fraksi n-heksan, fraksi etil asetat dan fraksi air berturutan sebesar 21,88; 10,90; 3,96 dan 52,00%. Hasil uji aktivitas antibakteri menunjukkan fraksi n-heksan, etil asetat dan air memiliki aktivitas antibakteri terhadap Staphylococcus epidermidis. Fraksi etil asetat merupakan fraksi teraktif dibandingkan fraksi yang lain dengan diameter daya hambat terbesar pada konsentrasi 20% sebesar 23,33 mm. Fraksi etil asetat mengandung senyawa flavonoid, alkaloid, saponin, dan terpenoid yang bertanggung jawab terhadap aktivitas antibakteri. Hasil penelitian dengan metode dilusi menunjukkan bahwa Konsentrasi Bunuh Minimum fraksi etil asetat adalah 5%. Kata kunci: herba ceplukan (Physalis angulata L.), fraksinasi, Staphylococcus epidermidis, antibakteri.
The use of aromatherapy was very well known in the community. The best-selling and quickly accepted product was aromatherapy oil. The benefits of aromatherapy were not only for health, but also for calming the mind and relaxation. Empowerment of PKK as members of the community and still classified as productive workforce was very important to do, aiming to foster awareness and independence in doing business, while expanding employment to increase family income in an effort to achieve a happy and prosperous family. In relation to efforts to foster and develop the potential of families and regions, it can be done through various alternative activities, including training in making aromatherapy oil product. The purpose of this community service program was to empower mothers and women through training and assistance in aromatherapy oil production. The target of this activity was PKK RW 04 Mojosongo Village, Jebres District, Surakarta City. The output of this activity was improving the skills of the community to produce aromatherapy wind oil and motivating business opportunities for PKK mothers. The method used in this service was training and assistance in aromatherapy oil production.
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