A new 4-h Yeast Identification Panel (YIP; Baxter-MicroScan, W. Sacramento, Calif.) was compared with the API 20C Yeast Identification System (Analytab Products, Inc., Plainview, N.Y.) in the identification of recent clinical yeast isolates. The YIP had a 94% correlation (288 of 306) in identifying 22 species within the genera Candida, Hansenula, Pichia, Rhodotorula, Saccharomyces, and Torulopsis. Correlation dropped to 65% for those species within the genera of slower growing yeasts, i.e., Blastoschizomyces spp., Crpytococcus spp., Geotrichum spp., Hyphopichia spp., Phaeococcomyces spp., Prototheca spp., and Trichosporon spp. Overall correlation with the API 20C was 92% (365 of 401) for those taxa included in the data base and 85% (373 of 437) for all yeasts encountered in the study. There were 36 (8.2%) discrepant identifications, which were due in part to the limited data base. Expansion of the data base plus the easy inoculation, reading, and rapid results of the YIP should make it an excellent method for yeast identification.

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Objective: To investigate the extent, distribution and sequence analysis of bla_CTX-M genes carried by Escherichia coli isolated from patients admitted to all government hospitals in Kuwait. Methods: Extended-spectrum β-lactamase (ESBL)-producing E. coli isolates were collected from the 8 major hospitals in Kuwait. CTX-M ESBLs were analyzed by PCR and sequenced. Clonality of the positive isolates was determined for genetic relatedness using pulsed-field gel electrophoresis (PFGE) with XbaI digestion of the genomic DNA. Results: Of the 136 ESBL-positive isolates, 106 (77.9%) harbored bla_CTX-M genes. Among these, bla_CTX-M-15 was the most frequent with a prevalence rate of 84.1%, followed by bla_CTX-M-14 (6.8%), bla_CTX-M-14b (5.7%) and bla_TOHO-1 (3.4%). Ninety-three (87.7%) were isolated from Kuwaiti (35.9%), Egyptian (31.1%) and Indian (20.8%) nationals; the majority of isolates positive for bla_CTX-M-15 were mainly from these 3 nationalities. PFGE analysis did not demonstrate any clustering of positive isolates in any particular hospital. Conclusion: This study confirms an explosive emergence of CTX-M-15 β-lactamase among E. coli isolates in Kuwait and shows that the strains were clonally heterogeneous with no evidence of inter- or intra-hospital spread. Thus Kuwait may represent an important source of CTX-M-15-positive E. coli.

The enterobacteriaceae, especially Escherichia coli and Klebsiella spp., as well as Acinetobacter spp., are important agents of nosocomial infections in hospitalized patients. A total of 460 Gram-negative bacteria (GNb), were investigated for their susceptibility to tigecycline and 9 other antibiotics by the etest. ESBL production was inferred from ESBL etest phenotypes. All the GNb, including the ESBL-producers, were susceptible to tigecycline with MIC(90 )ranges of 0.25 to 2 microg/ml. Imipenem and meropenem were very active against ESBL and non-ESBL producers; mean MIC(90)s of 0.19 and 0.09 microg/ml and 0.05 microg/ml and 0.02 microg/ml, respectively. The MIC(90)s of imipenem and meropenem for the Acinetobacter spp. were 16 and >32 microg/ml, respectively with resistance rates of 64.3 and 66.1%. ESBL production was detected in 62% and 82.1% of the E. coli and K. pneumoniae isolates, respectively. Resistance to ciprofloxacin was higher among the ESBL-producing strains of E. coli and K. pneumoniae than the non-ESBL producers. Comparatively, tigecycline had excellent in vitro activities against ESBL-producing enterobacteriaceae and demonstrated superior activity against Acinetobacter spp. Increasing ESBL production and resistance to ciprofloxacin and gentamicin in enterobacteriaceae require careful selection of empirical therapy. Tigecycline holds promise as an alternative choice of therapy for infections caused by ESBL-producing isolates and multi-drug resistant Acinetobacter spp.