Sequence Analysis of &lt;i&gt;bla&lt;/i&gt;&lt;sub&gt;CTX-M&lt;/sub&gt; Genes Carried by Clinically Significant &lt;i&gt;Escherichia coli&lt;/i&gt; Isolates in Kuwait Hospitals

Hashem, G. Al; Sweih, Noura Al; Jamal, W.; Rotimi, V.O.

doi:10.1159/000321242

Cited by 15 publications

(14 citation statements)

References 41 publications

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“…Unsurprisingly, urine samples were the major source (78.9%) of the resistant bacteria. This study also showed that while the majority of CTX-M-producing E. coli isolates have been found among Kuwaiti nationals, patients belonging to other nationalities have also contributed significantly (77). Of 16 randomly selected ESBL-producing K. pneumoniae isolates and 27 E. coli isolates, CTX-M-15 was found in 10 out of 11 bla CTX-M -positive K. pneumoniae and 19 out of 21 bla CTX-M -positive E. coli strains isolated between 2005 and 2006.…”

Section: Extended-spectrum and Ampc-type ␤-Lactamasessupporting

confidence: 55%

β-Lactamase Production in Key Gram-Negative Pathogen Isolates from the Arabian Peninsula

et al. 2013

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SUMMARY Infections due to Gram-negative bacilli (GNB) are a leading cause of morbidity and mortality worldwide. The extent of antibiotic resistance in GNB in countries of the Gulf Cooperation Council (GCC), namely, Saudi Arabia, United Arab Emirates, Kuwait, Qatar, Oman, and Bahrain, has not been previously reviewed. These countries share a high prevalence of extended-spectrum-β-lactamase (ESBL)- and carbapenemase-producing GNB, most of which are associated with nosocomial infections. Well-known and widespread β-lactamases genes (such as those for CTX-M-15, OXA-48, and NDM-1) have found their way into isolates from the GCC states. However, less common and unique enzymes have also been identified. These include PER-7, GES-11, and PME-1. Several potential risk factors unique to the GCC states may have contributed to the emergence and spread of β-lactamases, including the unnecessary use of antibiotics and the large population of migrant workers, particularly from the Indian subcontinent. It is clear that active surveillance of antimicrobial resistance in the GCC states is urgently needed to address regional interventions that can contain the antimicrobial resistance issue.

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Section: Extended-spectrum and Ampc-type ␤-Lactamasessupporting

confidence: 55%

β-Lactamase Production in Key Gram-Negative Pathogen Isolates from the Arabian Peninsula

et al. 2013

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“…The heterogeneous natures of these enzymes were reported by Al Hashem et al (16), and Khanna et al (23). The rapid dissemination of non-clonal multi-resistant CTX-M-1 producing E. coli isolates indicates the need for further investigations in medical centers.…”

Section: Discussionmentioning

confidence: 85%

“…Obviously, our results were analyzed based on the new 2010 CLSI interpretative criteria (10). A high incidence of ESBL-producers amongst E. coli was reported from India (79%), Pakistan (64%) and Kuwait (77.9%) (16)(17)(18). These studies indicate a high incidence of ESBL-producers amongst E. coli isolates in Asian countries.…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of bla CTX-M-1 Group Extended-Spectrum β-lactamase Genes in Escherichia coli Isolates From Tehran

Peerayeh

Eslami

Memariani

et al. 2013

Jundishapur J Microbiol

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Background:The emerge of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli caused problems in antibiotic treatments. The more than 50 variants of CTX-M type ESBLs enzymes are available worldwide. Rapid and discriminative typing methods are essential for epidemiological analysis of clinical CTX-M-producing isolates. Objectives: To assess the frequency and genetic diversity of extended-spectrum CTX-M β -lactamases in E. coli isolates from Tehran. Materials and Methods: During 2009During -2010 non-duplicate clinical isolates of E. coli were collected from five hospitals in Tehran. Antibacterial susceptibility was determined using disk diffusion and MIC methods. ESBL production was confirmed by Combined Disk and MIC. CTX-M encoding genes were identified by PCR, and blaCTX-M -carrying isolates were genotyped by rep-PCR. Results: A total of 140(70%) non-duplicate ESBL positive E. coli were determined. Imipenem and Amikacin were effective against 100% and 85% of all tested isolates respectively. Co-resistance of ESBL positive isolates to Erythromycin, Tetracycline, Co-trimoxazole and Ciprofloxacin were found in 93%, 75%, 63% and 43% of the strains, respectively. The rate of cefotaxime and ceftazidime resistant isolates dramatically decreased from 72% to 8.5% and 39% to 4.5% respectively, in the presence of clavulanic acid. Eighty nine (44.5%) E. coli isolates carried the blaCTX-M-1 group alleles. The levels of similarity of the rep-PCR fingerprints of blaCTX-M-1 isolates ranged from 40 to 90%. Conclusions: The rapid dissemination of non clonal multi-resistant CTX-M-1 producing E. coli isolates approved the need for further studies in our medical centers.

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“…In recent reports from the UAE and Kuwait, the proportions of Enterobacteriaceae isolates with the CTX-M genotype were 87 and 77.9%, respectively [16,17]. Lower figures have emerged from Saudi Arabia, with two recent reports showing bla CTX-M detection in 34 and 71% of Enterobacteriaceae isolates [14,18].…”

Section: Discussionmentioning

confidence: 99%

Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae: In vitro Susceptibility to Fosfomycin, Nitrofurantoin and Tigecycline

et al. 2012

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Objective: To assess the susceptibility trends of community-acquired extended-spectrum β-Iactamase (ESBL)-producing urinary isolates with particular reference to fosfomycin, nitrofurantoin and tigecycline. Materials and Methods: Seven hospitals across the United Arab Emirates participated in this study from June 2008 to March 2010. The antibiotic sensitivity of ESBL-producing uropathogens to a panel of antibiotics including tigecycline, fosfomycin and nitrofurantoin was assessed. The Hyplex ESBL identification system (h-ES-ID) was used for genotypic identification. Results: Two hundred and ninety-two ESBL-producing Enterobacteriaceae isolates were identified during the study period. Of these, 182 (62%) were urinary isolates and comprised of Escherichia coli: 149 (81.9%), Klebsiella pneumoniae: 30 (16.5%) and Proteus mirabilis: 3 (1.6%). Of the 182 urinary isolates, 179 (98.3%) were from patients with community onset urinary tract infections. The h-ES-ID system identified 172 (94.5%) of the urinary isolates as CTX-M positive. All isolates were susceptible to imipenem and meropenem. Over half of the isolates showed resistance to gentamicin (98; 53.8%), trimethoprim-sulfamethoxazole (139; 76.4%) and ciprofloxacin (143; 78.6%). Sensitivity to nitrofurantoin and fosfomycin was 90 and 100%, respectively. Two CTX-M-positive K. pneumoniae isolates with tigecycline resistance (MIC >4 µg/ml) were identified. Conclusion: There is dissemination of CTX-M ESBL-producing urinary pathogens into the community. Fosfomycin and nitrofurantoin were active against ESBL-positive uropathogens, and emergence of tigecycline resistance needs close monitoring.

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Sequence Analysis of <i>bla</i><sub>CTX-M</sub> Genes Carried by Clinically Significant <i>Escherichia coli</i> Isolates in Kuwait Hospitals

Cited by 15 publications

References 41 publications

β-Lactamase Production in Key Gram-Negative Pathogen Isolates from the Arabian Peninsula

β-Lactamase Production in Key Gram-Negative Pathogen Isolates from the Arabian Peninsula

High Prevalence of bla CTX-M-1 Group Extended-Spectrum β-lactamase Genes in Escherichia coli Isolates From Tehran

Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae: In vitro Susceptibility to Fosfomycin, Nitrofurantoin and Tigecycline

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