With only a single class of antiviral drugs existing for treatment of influenza (neuraminidase inhibitors), the search for novel effective compounds is urgently needed. We evaluated a low molecular mass compound, enisamium iodide (FAV00A), against influenza virus infections in primary differentiated normal human bronchial epithelial (NHBE) cells, and in ferrets. FAV00A (500 µg/ml) markedly inhibited influenza virus replication and reduced viral M-gene expression in NHBE cells. Treatment of ferrets with FAV00A (200 mg/kg once daily for 7 days) initiated 24 h after inoculation with 105 TCID50 of influenza A/Wisconsin/67/2005 (H3N2) virus resulted in a significant decrease in virus titers in the upper respiratory tract. Our data show that FAV00A exhibits an antiviral effect against influenza virus in NHBE cells and provides some benefits in a ferret model. Thus, further Keywords: antiviral agents; enisamium iodide; influenza virus; MDCK cells; NHBE cells; ferrets.

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Kinetics of the UV-induced (lambda = 254 nm) inactivation of phage MS2 is studied for stirred and non-stirred solutions with optical densities ranging from 0.06 to 2.1. The extent of inactivation exhibits exponential dependence on irradiation time for stirred solutions thus indicating the homogeneity of the phage population in respect to photosensitivity of infectivity. Irradiation of the same solution without stirring results in deviation of survival curves from exponential ones (the so called "tailing"), being more pronounced the larger the optical density of the layer under irradiation and degree of inactivation. Such a deviation is conditioned by non-uniform light absorption by virions in solution in the absence of stirring. The inactivation kinetics upon UV-irradiation of the stirred and non-stirred phage solutions is rather accurately expressed by respective equations thus enabling the choice of rational conditions for a given depth of phage inactivation to be performed.

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