High-impact exercise has been considered an important method for treating bone loss
in osteopenic experimental models. In this study, we investigated the effects of
osteopenia caused by inactivity in femora and tibiae of rats subjected to jump
training using the rat tail suspension model. Eight-week-old female Wistar rats were
divided into five groups (n=10 each group): jump training for 2 weeks before
suspension and training during 3 weeks of suspension; jump training for 2 weeks
before suspension; jump training only during suspension; suspension without any
training; and a control group. The exercise protocol consisted of 20 jumps/day, 5
days/week, with a jump height of 40 cm. The bone mineral density of the femora and
tibiae was measured by double energy X-ray absorptiometry and the same bones were
evaluated by mechanical tests. Bone microarchitecture was evaluated by scanning
electron microscopy. One-way ANOVA was used to compare groups. Significance was
determined as P<0.05. Regarding bone mineral density, mechanical properties and
bone microarchitecture, the beneficial effects were greater in the bones of animals
subjected to pre-suspension training and subsequently to training during suspension,
compared with the bones of animals subjected to pre-suspension training or to
training during suspension. Our results indicate that a period of high impact
exercise prior to tail suspension in rats can prevent the installation of osteopenia
if there is also training during the tail suspension.
ObjectivesEpidemiological studies have shown a relationship between long-term use of proton pump inhibitors and bone metabolism. However, this relationship has not yet become established. The aim of the present study was to analyze the mechanical properties and bone mineral density (BMD) of rats that were subjected to long-term omeprazole use.MethodsFifty Wistar rats weighing between 200 and 240 g were divided equally into five groups: OMP300 (omeprazole intake at a dose of 300 μmoL/kg/day); OMP200 (200 μmoL/kg/day); OMP40 (40 μmoL/kg/day); OMP10 (10 μmoL/kg/day); and Cont (control group; intake of dilution vehicle). The solutions were administered for 90 consecutive days. After the rats had been sacrificed, their BMD, the mechanical properties of the dissected femurs and their serum Ca++ levels were analyzed.ResultsThe BMD of the OMP300 group was lower than that of the controls (p = 0.006). There was no difference on comparing the OMP200, OMP40 and OMP10 groups with the controls. The maximum strength and rigidity of the femur did not differ in the experimental groups in comparison with the controls. The OMP300 group had a statistically lower serum Ca++ concentration than that of the controls (p = 0.049), but the other groups did not show any difference in relation to the controls.ConclusionDaily intake of 300 μmoL/kg/day of omeprazole decreased the BMD of the femur, but without changes to the rigidity and strength of the femur in adult rats.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.