Fixation CO 2 with microalgae is a potential promising technique to reduce CO 2 emission for its various advantages. In this work, we develop a microalgal fixation CO 2 system to capture CO 2 from actual flue gas. At first, we evolved a strain Scenedesmus obliquus, named S. obliquus WUST4, with a UV mutagenesis technique, which can endure actual flue gas. The S. obliquus WUST4 can tolerate high concentration CO 2 , and possesses high CO 2 fixation ability. Then, we developed a pilot scale microalgal fixation CO 2 system to capture CO 2 from actual flue gas with the evolved S. obliquus WUST4. The flue gas was discharged from the combustion chamber in a coke oven. A 100 L air-lift photobioreactor was built in this system. In the pilot scale system, CO 2 can be captured from the actual flue gas by the S. obliquus WUST4. The CO 2 removal ratio can reach 67% with the optimal operation conditions. This provides an efficient technique for CO 2 capture. ' EXPERIMENTAL SECTIONMicroalgae Strain and Culture Conditions. S. obliquus was used in this work. It was obtained from Institute of Hydrobiology

As an important organic compound, chiral alcohols are the key chiral building blocks to many single enantiomer pharmaceuticals. Asymmetric reduction of the corresponding prochiral ketones to produce the chiral alcohols by biocatalysis is one of the most promising routes. Asymmetric reduction of different kinds of non-natural prochiral ketones catalyzed by various plants tissue was studied in this work. Acetophenone, 4'-chloroacetophenone and ethyl 4-chloroacetoacetate were chosen as the model substrates for simple ketone, halogen-containing aromatic ketone and beta-ketoesters, respectively. Apple (Malus pumila), carrot (Daucus carota), cucumber (Cucumis sativus), onion (Allium cepa), potato (Soanum tuberosum), radish (Raphanus sativus) and sweet potato (Ipomoea batatas) were chosen as the biocatalysts. It was found that these kinds of prochiral ketoness could be reduced by these plants tissue with high enantioselectivity. Both R- and S-form configuration chiral alcohols could be obtained. The e.e. and chemical yield could reach about 98 and 80% respectively for acetophenone and 4'-chloroacetophenone reduction reaction with favorable plant tissue. And the e.e. and yield for ethyl 4-chloroacetoacetate reduction reaction was about 91 and 45% respectively.

Anaerobic co-digestion of dairy manure, chicken manure with corn stalks were investigated at 15, 35 and 55 °C. Two trials were carried out, one based on co-digestion of dairy or chicken manure alone with corn stalks at ratios of 1:1, 2:1 and 3:1, the other based on co-digestion of dairy and chicken manure together with corn stalks on the premise of the optimal ratio between manure and stalks. In the first trial, the analysis based on the biogas and methane yields showed that co-digestion of dairy or chicken manure with corn stalks at 2:1 both had higher fermentabilities than other ratios. In the second trial, at 15 °C and 35 °C, various ratios of co-digestion of dairy and chicken manure together with corn stalks were all higher than co-digestion of two kinds of manure alone with corn stalks and maximum biogas and methane yields were obtained at the C/N ratios from 25 to 28. At 55 °C, only at the mixture ratios of 1.6:0.4:1, co-digestion of dairy and chicken manure together with corn stalks were higher than co-digestion of dairy manure with corn stalks, indicating the optimal C/N ratios from 28 to 30 in biogas and methane production. Based on regression analysis, maximum biogas and methane yields were found at 49.9 °C and 46.9 °C with the ratios of dairy, chicken manure and corn stalks at 1.14:0.86:1 and 0.74:1.26:1, respectively.

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China is a large agricultural country that has abundant biomass resource which can be generated biogas for heating and lighting. To improve biogas production efficiency of human excreta, which is a main kind of material in household biogas use, a laboratory-scale simulated experimental using self-designed constant temperature anaerobic fermentation equipment was conducted in our study. The results showed that increase of temperature can improve biogas generation efficiency of human excreta, and the other way is to mix the human excreta with other materials especially with the high C materials. Compare with the high costing of increase temperature, co-digestion with wheat straw has obvious advances: the crop straw can easily obtain and both wastes can be handled in the anaerobic fermentation process. The co-digestion developed in our study may be applicable to human excreta with other crop straws such as corn straw for biogas production.