Anaerobic co-digestion of dairy manure, chicken manure with corn stalks were investigated at 15, 35 and 55 °C. Two trials were carried out, one based on co-digestion of dairy or chicken manure alone with corn stalks at ratios of 1:1, 2:1 and 3:1, the other based on co-digestion of dairy and chicken manure together with corn stalks on the premise of the optimal ratio between manure and stalks. In the first trial, the analysis based on the biogas and methane yields showed that co-digestion of dairy or chicken manure with corn stalks at 2:1 both had higher fermentabilities than other ratios. In the second trial, at 15 °C and 35 °C, various ratios of co-digestion of dairy and chicken manure together with corn stalks were all higher than co-digestion of two kinds of manure alone with corn stalks and maximum biogas and methane yields were obtained at the C/N ratios from 25 to 28. At 55 °C, only at the mixture ratios of 1.6:0.4:1, co-digestion of dairy and chicken manure together with corn stalks were higher than co-digestion of dairy manure with corn stalks, indicating the optimal C/N ratios from 28 to 30 in biogas and methane production. Based on regression analysis, maximum biogas and methane yields were found at 49.9 °C and 46.9 °C with the ratios of dairy, chicken manure and corn stalks at 1.14:0.86:1 and 0.74:1.26:1, respectively.

China is a large agricultural country that has abundant biomass resource which can be generated biogas for heating and lighting. To improve biogas production efficiency of human excreta, which is a main kind of material in household biogas use, a laboratory-scale simulated experimental using self-designed constant temperature anaerobic fermentation equipment was conducted in our study. The results showed that increase of temperature can improve biogas generation efficiency of human excreta, and the other way is to mix the human excreta with other materials especially with the high C materials. Compare with the high costing of increase temperature, co-digestion with wheat straw has obvious advances: the crop straw can easily obtain and both wastes can be handled in the anaerobic fermentation process. The co-digestion developed in our study may be applicable to human excreta with other crop straws such as corn straw for biogas production.