SummaryYeast biomass (Saccharomyces sp.) produced in local breweries as a by-product was utilized in this study. Percent proximate composition, amino acid composition, and protein nutritive value were determined for the yeast cell biomass (YC), a sodium perchlorate extracted and isoelectrically precipitated protein concentrate (P-PC), and a sodium trimetaphosphate treated extract followed by isoelectrical precipitation (TMP-PC). Protein concentrates averaged 75% protein as compared to 48.5% in the yeast biomass. Precipitation of the protein in the presence of either sodium perchlorate or sodium trimetaphosphate was reduced to 71% and 51% of the cell RNA content, respectively. Protein nutritive value was 70% of casein when measured by the protein efficiency ratio (PER), and over 90% of casein when net protein utilization (NPUa) was the criteria of evaluation.

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Iron deficiency anemia is the world most prevalent nutritional problem. To investigate anemia prevalence and its relation to nutritional status, 526 children and adolescents, 284 males and 242 females, aged 7 to 14 years old, were evaluated. The studied subjects take part in a program of the Social Action Department of the city of Londrina, state of Paraná, and attend Public Educational Centers which provides them three daily meals and pedagogic assistance. These individuals belong to deprived areas in the city outskirts, where the per capita income is lower than US$500 a year. The hemoglobin dosage was determined by digital puncture and read by Hemocue portable photometer. Anemia prevalence was verified in 41.3% of the assessed population, with no significant statistical differences between males and females (p=0.412). No association between anemia and malnutrition was found. In conclusion, high prevalence of anemia was observed in this population.