Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process.

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O presente trabalho relata o protocolo anestésico utilizado em dois cães submetidos à cirurgia de ressecção e reconstrução da parede torácica devido a neoplasmas, no Hospital Veterinário Roque Quagliato do Centro Universitário das Faculdades Integradas de Ourinhos, São Paulo. Uma cadela poodle de 10 anos e um cão rottweiler de um ano e três meses foram submetidos à anestesia geral com isofluorano, bloqueio intercostal de, respectivamente, seis e sete espaços intercostais com lidocaína 2% e bupivacaína 0,5% e infusão contínua de morfina (0,1 mg/kg/h), lidocaína (50 μg/kg/minuto) e midazolam (0,1 mg/ kg/h). No período pós-operatório imediato, ambos receberam administração peridural de morfina 0,2 mg/kg e fentanila 2 μg/kg diluídos com NaCl 0,9% para 0,3 mL/kg. Um tubo de analgesia foi deixado durante as 48 horas subsequentes na ferida cirúrgica para administração de lidocaína e bupivacaína em total de uma dose tóxica por dia, fracionada para cinco aplicações. A prescrição analgésica pós-operatória constituiu-se de tramadol 4 mg/kg e dipirona 25 mg/kg a cada oito horas durante sete dias, juntamente com meloxicam 0,1 mg/kg a cada 24 horas durante três dias. Os animais recuperaram-se tranquilamente e não foram observados sinais de dor ao longo dos sete dias subsequentes. Recomenda-se o protocolo relatado para cirurgias de ressecção da parede torácica em cães.