Among human influenza viruses, strain A/H3N2 accounts for over a quarter of a million deaths annually. Antigenic variants of these viruses often render current vaccinations ineffective and lead to repeated infections. In this study, a computational model was developed to predict antigenic variants of the A/H3N2 strain. First, 18 critical antigenic amino acids in the hemagglutinin (HA) protein were recognized using a scoring method combining phi (݊) coefficient and information entropy. Next, a prediction model was developed by integrating multiple linear regression method with eight types of physicochemical changes in critical amino acid positions. When compared to other three known models, our prediction model achieved the best performance not only on the training dataset but also on the commonly-used testing dataset composed of 31878 antigenic relationships of the H3N2 influenza virus.

MicroRNAs (miRNAs) are a class of short, non-coding RNA that play regulatory roles in a wide variety of biological processes, such as plant growth and abiotic stress responses. Although several computational tools have been developed to identify primary miRNAs and precursor miRNAs (pre-miRNAs), very few provide the functionality of locating mature miRNAs within plant pre-miRNAs. This manuscript introduces a novel algorithm for predicting miRNAs named miRLocator, which isbased on machine learning techniques and sequence and structural features extracted from miRNA:miRNA* duplexes. To address the class imbalance problem (few real miRNAs and a large number of pseudo miRNAs), the prediction models in miRLocator were optimized by considering critical (and often ignored) factors that can markedly affect the prediction accuracy of mature miRNAs, including the machine learning algorithm and the ratio between training positive and negative samples. Ten-fold cross-validation on 5854 experimentally validated miRNAs from 19 plant species showed that miRLocator performed better than the state-of-art miRNA predictor miRdup in locating mature miRNAs within plant pre-miRNAs. miRLocator will aid researchers interested in discovering miRNAs from model and non-model plant species.

Taxi waiting times is an important criterion for taxi passengers to choose appropriate pick-up locations in urban environments. How to predict the taxi waiting time accurately at a certain time and location is the key solution for the imbalance between the taxis’ supplies and demands. Considering the life schedule of urban residents and the different functions of geogrid regions, the research developed in this paper introduces a spatio-temporal schedule-based neural network for urban taxi waiting time prediction. The approach integrates a series of multi-source data from taxi trajectories to city points of interest, different time frames and human behaviors in the city. We apply a grid-based and functional structuration of an urban space that provides a lower-level data representation. Overall, the neural network model can dynamically predict the waiting time of taxi passengers in real time under some given spatio-temporal constraints. The experimental results show that the granular-based grids and spatio-temporal neural network can effectively predict and optimize the accuracy of taxi waiting times. This work provides a decision support for intelligent travel predictions of taxi waiting time in a smart city.

The prediction of the demand for raw materials is of vital importance to modern industries. Most studies are based on traditional regression, linear programming, and other methods. Previous studies have often overlooked the characteristics of the sugar raw materials business and the influence of time factors on raw material demand, resulting in limited prediction accuracy. How to accurately predict the demand for sugar raw materials is one of the key issues for intelligent management. In view of the above problems, combined with the characteristics of the supply and demand cycle of sugar raw materials, this paper aims to predict the demand for raw materials based on their supply and demand in a real sugar company by optimizing the Elman neural network through the modified cuckoo search (MCS) algorithm with temporal features. This study proposes a temporal feature-correlation cuckoo search–Elman neural network (TMCS-ENN) for predicting the demand for sugar raw materials. The experimental results show that the accuracy of the TMCS-ENN model reaches 93.89%, a better performance than that achieved by existing models. Therefore, the study model effectively improves the accuracy of the demand forecast of sugar raw materials for companies. This output will be helpful for improving the production efficiency and automation level, as well as reducing costs.

Work zones, being a critical component of roadway transportation systems, can benefit greatly from computer vision-enabled roadway infrastructures, specifically in connected vehicle (CV) environments. Connected infrastructures, such as roadside units (RSU) and on-board units (OBU), can greatly improve the environmental awareness and safety of CVs driving through a work zone. The contribution of this paper lies in developing a vision-based approach to generate work zone safety messages in real time, utilizing video streams from roadside monocular traffic cameras that can be used by CV work zone safety apps on mobile devices to reliably navigate through a work zone. A monocular traffic camera calibration method is proposed to establish the accurate mapping between the image plane and Global Position System (GPS) space. Real test scenarios show that our algorithm can precisely and effectively locate work zone boundaries from a monocular traffic camera in real time. We demonstrate the capabilities and features of our system through real-world experiments where the driver cannot see the work zone. End-to-end latency analysis reveals that the vision-based work zone safety warning system satisfies the active safety latency requirements. This vision-based work zone safety alert system ensures the safety of both the worker and the driver in a CV environment.