(Z)-Cinnamate derivatives are prevalent in natural bioactive products and in organic synthesis. Herein, we report a practical approach toward the efficient synthesis of (Z)-cinnamate derivatives via visible-light-driven isomerization. When E-isomers of cinnamate derivatives were irradiated with blue light in the presence of 1 mol% Ir2(ppy)4Cl2 (ppy = 2-phenylpyridine), Z-isomers were readily obtained in good yields. This strategy allows the large-scale synthesis of (Z)-cinnamate derivatives with simple purification. This convenient, mild, and green synthetic methodology was subsequently applied to the synthesis of coumarins.
Metal–organic
frameworks (MOFs), as a kind of poriferous
nanoparticle, are promising candidates for enzyme immobilization to
enhance their stability and reusability. However, most MOFs could
not specifically immobilize enzymes and regenerate easily, which inevitably
leads to serious high consumption and environmental pollution. In
this study, renewable and magnetic MOFs were first constructed to
specially immobilize His-tagged enzymes from the cell lysates without
purification. The immobilized β-glucuronidase exhibited wider
pH adaptability and temperature stability. The relative activity of
immobilized β-glucuronidase was still maintained at ∼80%
after eight cycles. Importantly, after simple treatment, the immobilization
capacity of regenerated MOFs after simple treatment was restored to
more than 90% in the first three times. The specific magnetic MOFs
were proven to be an efficient and renewable platform for one-step
immobilization and purification of His-tagged enzymes, showing great
potential in industrial applications of nanotechnology and biocatalysis.
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