In this study, it was aimed to investigate the effects of both using curcumin and microencapsulation method on in vitro release behaivour of chia seed oil and its antioxidant potential during simulated gastrointestinal (GI) tract. Maltodextrin (MD) and gum Arabic (GA) was used as wall materials for freeze dried capsules. Sample 6, having 1:3 MD to GA ratio, 1:5 chia seed oil to wall material ratio and 40% total dry matter content, was found to have the optimum results in terms of emulsion stability (CI% = 0), zeta potential (−32.2 ± 0.8 mV) and size distribution (600 ± 8 nm). Moreover, release profiles of encapsulated chia seed oil samples were evaluated to determine if curcumin addition has any significant effect. The results revealed that curcumin addition decreased the release of chia seed oil from 44.6% to 37.2%. On contrary, it increased total phenolic content of in fraction of intestine to 22 mg gallic acid equivalents (GAE)/L.

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The main source of long-chain (LC) omega-3 fatty acids are oily fish, fish oils, and some algae extracts, and they have numerous health benefits such as reducing the risk of cancer, heart disease, inflammation, arthritis, diabetes, and high cholesterol (Hong et al., 2011;Mazza et al., 2007;Saini & Keum, 2018). Eicosapentaenoic acid (EPA) is a 20-carbon-long omega-3 fatty acid, and the most important of EPA's main functions is its use to create signaling molecules called eicosanoids which can reduce inflammation (Siriwardhana et al., 2012).

Echium oil (EO) contains substantial amounts of omega-3 fatty acids, which are important because of their benefits to human health. However, they are prone to oxidation. The aim of this study was to obtain the optimum conditions of microencapsulation of EO using spray drying by applying the response surface methodology (RSM). Central composite circumscribed design (CCC) was employed with a ratio of maltodextrin (MD):EmCap modified starch (MS) (80−90%, w/ w), oil concentration (15−25%, w/w), and homogenization speed (5− 15 × 10 3 rpm) as independent variables affecting droplet size (μm) and viscosity (Pa•s), which were chosen as responses for the emulsification process. The results revealed that the emulsion conditions containing MD:MS (89.7%:10.3%, w/w), oil concentration of (16.0%), and homogenization speed at (14.8 × 10 3 rpm) were found to be the optimum conditions. Furthermore, for encapsulation, CCC was employed with inlet temperature of 140−180 °C, air flow of 20− 30%, and pump rates of 15−25% as independent variables. Total yield (%) and encapsulation efficiency (%) were chosen as responses for the encapsulation process. On the other hand, optimum conditions for encapsulation were as follows: inlet temperature of 140 °C, airflow rate of (30%) 0.439 m 3 /h, pump rate of (15%) 4.5 mL/min with respect to selected responses.

Omega-3 fatty acid consumption is getting more and more common due to their positive impacts on human health. Since consumers cannot get their omega-3 needs from natural sources, omega-3 supplements play an essential part of the diet. Omega-3 fatty acids are highly susceptible to oxidation; thus, storage conditions affect the quality of these fatty acids. Supplement form is also another factor, which is critical for stability. In this project, it is aimed to compare the effects of different forms (capsule, chewable nd syrup) and packaging types of fish oil supplements on fatty acid composition, oxidative stability and sensory properties when stored at room temperature and in dark conditions. Moreover, the effect of consumer behavior regarding the recommended usage after the bottles are opened were assesed during storage study. It was observed that, syrup forms are more susceptible to oxidation than capsule and chewable forms. Moreover, capsule forms complied more with limit values during storage and better protected according to the sensory evaluation scores. Fish oil samples belonging to the same company but provided from different stores showed significant differences in some of the sensory attributes which is an indicator of non-standard raw material, ingredient or processing.