Once the initial density, D1, is noted, a single piece of freshly isolated mammalian tissue is analyzed for water and fat. Knowing the densities of water and fat, the tissue's fat-free density, D2, and the dry, fat-free density, D3, can be computed. Among the various types of soft tissues, since D3 is similar, a simple relationship is shown to exist between the fat-free water content and the corresponding density. The biological extremes in a water:fat:protein system thus operate in such a way that the quantity of fat can be predicted from an equation containing three variables, i.e. the initial weight and density of a tissue and its quantity of water. This is of interest in human body composition and also to those who desire to make a sequence of determinations on a single piece of tissue without having to submit a portion thereof to direct analysis of fat, e.g. to express tissue electrolytes and water content on the fat-free basis. Submitted on June 15, 1959

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Body Corn position Branch, Bioenergetics Division. 8 times (6), which is not unusual since there is some decalcification of the bones during starvation (9). No red blood cells, albumin, or casts have been reported in urine sediments (3). It has been observed for many years that acute starvation has never been associated with the development of any

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