ABSTRAKKonsumsi Epigallocatechin Gallate (EGCG) teh hijau dilaporkan banyak bermanfaat pada upaya peningkatan kesehatan, seperti pembakaran lemak, mencegah obesitas dan sensitifitas insulin. Sehingga teh hijau (Camelia sinensis) dari klon GMB4 dapat dikembangkan sebagai agen terapeutik potensial untuk obesitas dan resistensi insulin. Penelitian ini bertujuan untuk membuktikan Epigallocatechin Gallate (EGCG) teh hijau dapat menghambat peningkatan kadar SREBP-1 jaringan adiposa dan resistensi insulin pada tikus galur wistar jantan yang diberi diet tinggi lemak. Penelitian ini dilakukan secara invivo dengan pemeliharaan hewan coba selama 8 minggu yang dibagi dalam lima kelompok perlakuan: (1) kelompok kontrol (-) dengan pemberian diet pakan standart, (2) kelompok kontrol (+) dengan pemberian diet tinggi lemak, (3)Pemberian diet tinggi lemak+EGCG 1mg/kgBB, (4) Pemberian diet tinggi lemak+EGCG 2 mg/kgBB, (5) Pemberian diet tinggi lemak+EGCG 8 mg/kgBB. Pakan tikus diberikan secara oral, sedangkan EGCG per sonde 1 x/hr. Metode yang digunakan dalam penelitian ini adalah ELISA untuk kadar insulin dan SREBP-1 jaringan adiposa dan spektrofotometri untuk glukosa darah puasa. EGCG menurunkan lemak viseral, kadar glukosa, kadar SREBP-1 dan resistensi insulin (HOMA-IR) (p<0,05). Penurunan kadar SREBP-1 secara signifikan sebesar 29,85%(p<0,05) pada dosis 8 mg/kgBB, sedangkan HOMA-IR menurun secara signifikan sebesar 33,89% (p<0,05) pada dosis 8 mg/kgBB. Terdapat hubungan antara kadar SREBP-1 dengan resistensi insulin (HOMA-IR) (p<0,05). Hasil penelitian ini membuktikan bahwa EGCG mampu menghambat resistensi insulin yang mungkin melalui penurunan adipo/lipogenesis SREBP-1 jaringan adiposa, dengan bukti adanya penurunan kadar SREBP-1 jaringan adiposa dan lemak viseral.Kata Kunci: EGCG, glukosa, HOMA-IR, insulin, SREBP-1 jaringan adipose, tikus diet tinggi lemak ABSTRACT Consumption of epigallocatechin gallate (EGCG) of green tea were reported to have much benefit in improving health, such as increased fat oxidation, prevent obesity and improve insulin sensitivity. EGCG of green tea (Camelia sinensis) from GMB4 clone may serve as a potential therapeutic agent for obesity and insulin resistance.This study investigated the effect isolat Epigallocatechin Gallate (EGCG) from green tea inhibit increasing adipose tissue sterol regulatory element-binding protein 1( SREBP-1 ) and insulin resistance in male rats with high fat diet. Wistar male rats were fed a diet high in fat for 2 months from 6-8 weeks of age and determination of the object of research with completely randomized design with five treatments, namely (1) rats with standard feed diet (2) rats with a diet high in fat, (3) rats with high-fat diet + EGCG 1mg/kgBW, (4) rats with high-fat diet + EGCG 2 mg/kgBW, (5) rats with high-fat diet + EGCG

Background:The present study was undertaken to investigate the hepatoprotective effect of Aloe vera against side effect of antituberculosis drug.Material and methods:Twenty-five rats will be divided into five groups, namely the control group (without any treatment), the group of rats treated with anti-tuberculosis drugs, and a group of rats were treated antituberculosis drugs and got Aloe vera extract at a dose of 40; 80; and 120 mg/kg body weight. Antituberculosis drugs are isoniazid and rifampicin a dose of 50 mg/kg body weight.Results:Antituberculosis treated group showed significantly increase levels of TNF-a, the percentage of NK cells and the number of Th17 cells compared with the control group (p < 0.05). All doses of Aloe vera reduce levels of TNF-a compared with the antituberculosis group (p < 0.05), although it has not yet reached levels comparable to the control group (p > 0.05). Aloe vera at first and the third dose lower the number of NK cells compared to the antituberculosis group, although it has not yet reached a significant difference (p > 0.05). The first dose of Aloe vera was significantly decreased the percentage of Th17 cells compared to the antituberculosis drug group (p < 0.05), although it has not yet reached levels comparable to the control group (p > 0.05).Conclusions:It was concluded that administration of Aloe vera can suppress the production of TNF-a and the percentage of Th17 cells as a result of antituberculosis drug administration. Thus, Aloe vera can be a useful alternative to natural materials in the successful treatment of tuberculosis through the inhibition of side effect.

Purpose: To evaluate the effects of Citrus aurantifolia and Cinnamomum burmannii extracts (Jermanis) on the number of pancreatic β-cells and levels of superoxide dismutase (SOD), low-density lipoprotein (LDL), and transforming growth factor-beta (TGF-β) in β-cells. Methods: Various doses of a combination of extracts of C. aurantifolia and C. burmannii were given orally to 25 male Wistar rats (Rattus norvegicus) every 2 h for 30 days. Doses of 100, 300 and 500 mg/kg/day C. aurantifolia extract were considered low, medium and high doses, respectively, while, 200, 400 and 800 mg/kg/day C. burmannii extract were considered low, medium and high doses, respectively. LDL and SOD levels in blood serum were analyzed spectrophotometrically. TGF-β expression was evaluated by immunohistochemistry. Pancreatic tissue sections (diameter of a β-cell) were evaluated by light microscopy after hematoxylin and eosin staining. Results: The combination of C. aurantifolia and C. burmannii extracts increased SOD levels, TGF-β expression and the number of β-cells and decreased LDL levels in hyperglycemic rats. The results indicate that the highest doses of C. aurantifolia and C. burmannii increased the number of β-cells in the islets of Langerhans. The combined extracts of C. aurantifolia and C. burmannii significantly affected pancreatic cell regeneration. Conclusion: The combination of C. aurantifolia and C. burmannii extracts may be a promising alternative preventative medicine for management of diabetic complications in patients with hyperglycemia.

This study aims to evaluate the interaction of green tea active compounds with proteins related to mTOR signals. The in silico study uses SEA protein target software, DB strings, and AUTODOCK PYRX 9.5. There are twenty target proteins that can interact with the active compounds of green tea. Of the twenty proteins, only six proteins are connected to the mTOR pathway. Of the six proteins, one that is a regulator of mTOR inhibitors is PKCA. Epigallocatechin has the strongest interaction with PKCA 4ARA (-8 kcal / mol). Cianidanol has the strongest interaction with PKCA 3IW4 (-9.3 kcal / mol). To analyze the involvement of the autophagy, a docking between ULK1 and AMPK was conducted, and there was an interaction between ULK1 and AMPK (bond energy of -1446.11 kcal). For the interaction between mTOR and ULK1, the bond energy is -624.5 kcal. For active green tea compounds, the bonding energy is more positive than the mTOR bond with ULK1. It was concluded that the green tea active ingredient as an inhibitor control against mTOR through PKCA and ULK1-AMPK (autophagy pathway).

Tujuan pengabdian masyarakat ini adalah melakukan pemeriksaan kesehatan pada anak usia sekolah dasar di SD Plus Jombang.  Pemeriksaan kesehatan meliputi kesehatan dasar atau kesehatan umum dan pemeriksaan kesehatan penglihatan, kesehatan pendengaran dan kesehatan gigi  pada anak usia sekolah di SD Plus Jombang. Pelakasanaan kegiatan pengabdian masyarakat ini dilakukan pada tanggal 16 Juni 2022 yang terdiri dari kelas 1 sampai dengan kelas 5. Metode pelaksanaan kegiatan pengabdian masyarakat ini terdiri dari: 1) Tahap persiapan, 2) Tahap pelaksanaan dan 3) Tahap evaluasi. Tahap persiapan meliputi: Rapat koordinasi penentuan jadwal dan penentuan lokasi pemeriksaan, persiapan anggota Tim pelaksana, persiapan alat dan bahan dan setting untuk tempat dan prosedur  pelaksanaan. Tahap Pelaksanaan ywaitu pemeriksaan berurutan mulai dari meja pemeriksaan mata, meja pemeriksaan gigi, meja pemeriksaan pendengaran dan meja pemeriksaan umum. Selanjutnya tahap evaluasi berupa tahap4 evaluasi kegiatan mulai dari persiapan hingga akhir kegiatan. Hasil pelaksanaan kegiatan berjalan dengan lancar ada beberapa siswa tidak bisa mengikuti pemeriksaan