The simple, sensitive, and specific detection of hydrogen sulfide (H 2 S) is of great importance because of its crucial role in food safety, environmental pollution, and various pathological and physiological processes. Here, we reported activatable fluorescence nanoprobe-based quantum dots (QDs) for sensitive and selective monitoring of H 2 S in red wine, environmental water samples, and lysosome of live cancer cells. The nanoprobe was prepared through a strong electrostatic interaction between thioglycolic-acid-stabilized CdTe QDs and p-amino thiophenol capped silver nanoparticles (AgNPs) that resulted in the formation of the assembled nanostructure, called QD/AgNP nanocomplexes. The initial fluorescence of QDs was effectively quenched by the AgNPs because of the inner filter effect. Upon interaction with H 2 S, the strong etching ability of H 2 S to AgNPs could trigger the disassembly of QD/AgNP nanocomplexes and generate Ag 2 S on the surface of QDs, achieving a shell−core Ag 2 S/CdTe QDs with remarkable fluorescence as a result of the termination of inner filter effect. The aqueous solution studies displayed that the assembled QD/AgNP nanoprobe was sensitive to detect H 2 S, with a detection limit of 15 nM. In addition, this assembled QD/AgNP nanoprobe showed a high specificity toward H 2 S over other anions and biologically relevant species. The subsequent fluorescence imaging studies demonstrated that the assembled QD/AgNP nanoprobe exhibited high ability to enter into cellular lysosome and generated an enhancement fluorescence, which was used for endogenous H 2 S detection in lysosome of living cancer cells. This proposed nanoprobe revealed a more simple, rapid, time-saving, low-cost, sensitive, and selective process for monitoring of H 2 S in further environmental pollution, food safety, and clinical diagnosis of H 2 S-related diseases.

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