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Diabetes mellitus is one of the most prevalent metabolic disorders characterized by hyperglycemia due to impaired glucose metabolism. Overproduction of free radicals due to chronic hyperglycemia may cause oxidative stress, which delays wound healing in diabetic conditions. For people with diabetes, this impeded wound healing is one of the predominant reasons for mortality and morbidity. The study aimed to develop an Ocimum sanctum leaf extract-mediated green synthesis of titanium dioxide (TiO2) nanoparticles (NPs) and further incorporate them into 2% chitosan (CS) gel for diabetic wound healing. UV-visible spectrum analysis recorded the sharp peak at 235 and 320 nm, and this was the preliminary sign for the biosynthesis of TiO2 NPs. The FTIR analysis was used to perform a qualitative validation of the biosynthesized TiO2 nanoparticles. XRD analysis indicated the crystallinity of TiO2 NPs in anatase form. Microscopic investigation revealed that TiO2 NPs were spherical and polygonal in shape, with sizes ranging from 75 to 123 nm. The EDX analysis of green synthesized NPs showed the presence of TiO2 NPs, demonstrating the peak of titanium ion and oxygen. The hydrodynamic diameter and polydispersity index (PDI) of the TiO2 NPs were found to be 130.3 nm and 0.237, respectively. The developed TiO2 NPs containing CS gel exhibited the desired thixotropic properties with pseudoplastic behavior. In vivo wound healing studies and histopathological investigations of healed wounds demonstrated the excellent wound-healing efficacy of TiO2 NPs containing CS gel in diabetic rats.

Background: Cancer is a life-threatening global problem with high incidence rates. Prioritizing the prevention of cancer, chemopreventive agents have drawn much attention from the researchers. Background: This review focuses on the discussion of the progress in the development of chemopreventive agents and formulations related to the prevention of oral cancer. Methods: In this perspective, an extensive literature survey was carried out to understand the mechanism, control and chemoprevention of oral cancer. Different patented agents and formulations have also exhibited cancer preventive efficacy in experimental studies. This review summarizes the etiology of oral cancer and developments in prevention strategies. Results: The growth of oral cancer is a multistep activity necessitating the accumulation of genetic as well as epigenetic alterations in key regulatory genes. Many risk factors are associated with oral cancer. Genomic technique for sequencing all tumor specimens has been made available to help detect mutations. The recent development of molecular pathway and genetic tools has made the process of diagnosis easier, better forecast and efficient therapeutic management. Different chemical agents have been studied for their efficacy to prevent oral cancer and some of them have shown promising results. Conclusion: Use of chemopreventive agents, either synthetic or natural origin, to prevent carcinogenesis is a worthy concept in the management of cancers. Preventive measures are helpful in controlling the occurrence or severity of the disease. The demonstrated results of preventive agents have opened an arena for the development of promising chemopreventive agents in the management of oral squamous cell carcinoma.

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Background: Gallic acid is a natural phenolic compound found in several fruits and medicinal plants. It is reported to have several health-promoting effects including antioxidant, anti-inflammatory, diabetic, antineoplastic properties, gastrointestinal, neuropsychological, metabolic and cardiovascular disorders. Aims: The aim of the present work was to study the influence of formulation factors on the physicochemical properties of gallic acid-loaded chitosan nanoparticles in order to optimize the formulation. Methods: Active chitosan nanoparticles could be used to support the modification of gallic acid delivery. The nanoparticles were prepared by the emulsification solvent evaporation method using sonication. A 3-factor, 2-level BBD (Box-Behnken Design) was applied for exploring and optimizing the main effects, quadratic effects as well as interaction effects of the ingredients of the formulation on the performance of the nanoparticles. The responses like particle size, polydispersity index, zeta potential and encapsulation efficiency were also determined. Results: The concentration of gallic acid nanoparticles seems to be the most critical element affecting their properties. The concentration of chitosan was increased, which resulted in an increase in particle size. The optimised drug-loaded nanoparticles have a zeta potential of -5.2 mV due to their largely negative surface charge. Although the measured zeta potential was low, the nanoparticle dispersion remained stable, and no significant change in the shape or particle size of the gallic acid-loaded chitosan nanoparticles was seen after two weeks at 4°C. The gallic acid-loaded nanoparticles have a particle size of 230 nm. Gallic acid reduced the particle size after it was added. One probable explanation for this is that the loaded medicine increased the cohesive force of the hydrophobic contact, resulting in the size reduction. A formulation was optimised based on the acquired results, and the experimental values were comparable to the expected values. FTIR examination revealed that gallic acid-loaded chitosan nanoparticles displayed both hydrogen bond and ionic interactions, allowing for active agent encapsulation and augmentation. Conclusions: The overall results indicated that decreasing the chitosan concentration drug entrapement efficiency increases and gallic acid concentration was the main factor influencing particle size, while entrapment efficiency was predominantly affected by the chitosan concentration.