We demonstrate a hard-X-ray self-seeded (HXRSS) free-electron laser (FEL) at Pohang Accelerator Laboratory with an unprecedented peak brightness (3.2 × 10 35 photons/(s•mm 2 •mrad 2 •0.1%BW)). The self-seeded FEL generates hard X-ray pulses with improved spectral purity; the average pulse energy was 0.85 mJ at 9.7 keV, almost as high as in SASE mode; the bandwidth (0.19 eV) is about 1/70 as wide, the peak spectral brightness is 40 times higher than in self-ampli ed spontaneous emission (SASE) mode, and the stability is excellent with > 94% of shots exceeding the average SASE intensity. Using this self-seeded XFEL, we conducted serial femtosecond crystallography (SFX) experiments to map the structure of lysozyme protein; data-quality metrics such as R split , multiplicity, and signal-to-noise ratio for the SFX were substantially increased. We precisely map out the structure of lysozyme protein with substantially better statistics for the diffraction data and signi cantly sharper electron density maps compared to maps obtained using SASE mode.
In this paper, we implemented the conventional FAST and BRIEF algorithm as hardware on Zynq-7000 SoC Platform. Previous feature-based hardware accelerator is mostly implemented using the SIFT or SURF algorithm, but it requires excessive internal memory and hardware cost. The proposed FAST & BRIEF accelerator reduces approximately 57% of internal memory usage and 70% of hardware cost compared to the conventional SIFT or SURF accelerator, and it processes 0.17 pixel per clock.
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