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Background Extensive second language (L2) reading research has examined the effects of comprehension strategies on comprehension performance. Often ignored, however, is the potential impact of comprehension measures that differ in cognitive complexity, which might cause potential bias when interpreting the (in)effectiveness of comprehension strategies. Method With a total of 54 Chinese learners of English at college in China, this study examined whether the effect of the questioning strategy (answering what and why questions while reading) on L2 reading comprehension of expository texts might vary by comprehension measure (short‐answer and multiple‐choice questions). L2 readers' perception of the questioning strategy was also thematically analysed. Results Mixed‐effects modelling analyses revealed a significant interaction between the questioning strategy and comprehension measure, controlling for individual‐level differences in first language (L1) reading ability, L2 vocabulary size and topic familiarity. Using the questioning strategy significantly facilitated comprehension measured by short‐answer questions but not multiple‐choice questions. Three themes regarding L2 readers' perception of the questioning strategy were identified: memorising text details, understanding main ideas and disrupting reading fluency. Conclusion Evaluation of comprehension strategies needs to consider comprehension measures differing in response format and cognitive complexity. For positive effect to occur, there needs a match between cognitive processing triggered by a comprehension strategy and cognitive skills demanded to successfully complete a comprehension task.

By comparative analysis of histomorphology and AChE activity, the changes of physiological and biochemical parameters were determined in zebrafish embryos and larvae dealt with atrazine (ATR) at different concentrations (0.0001, 0.001, 0.01, 0.1, and 1 mg/L). This study showed that the development of the sarcomere and the arrangement of white muscle myofibers were affected by ATR significantly and the length of sarcomere shortened. Further analysis of the results showed that the AChE activity in juvenile fish which was treated with ATR was downregulated, which can indicate that the innervation efficiency to the muscle was impaired. Conversely, the AChE activity in zebrafish embryos which was treated with ATR was upregulated. A parallel phenomenon showed that embryonic primary sensory neurons (Rohon-Beard cells), principally expressing AChE in embryos, survived the physiological apoptosis. These phenomena demonstrated that the motor integration ability of the zebrafish was damaged by ATR which can disturb the development of sensory neurons and sarcomere and the innervations of muscle.

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