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Abstract. Tuheteru FD, Husna, Albasri, Arif A, Wulan SA, Kramadibrata K. 2019. Arbuscular mycorrhizal fungi associated with adaptive plants in gold mine tailing. Biodiversitas 20: 3398-3404. Arbuscular mycorrhizal fungi (AMF) are fungi from division Glomeremycota within the kingdom Fungi, which have a broad ecological range and symbiosis with a variety of vegetations, including vegetations in gold post-mining areas or gold tailings. The study of identifying types of AMF in gold tailings in Southeast Sulawesi is still limited. The purpose of this study was to identify the types of AMF in the rhizosphere of adaptive plants in gold tailings land in Bombana District, Southeast Sulawesi. Soil samples were collected from adaptive plants in Perseroan Terbatas (PT) Panca Logam Makmur, Bombana District, Southeast Sulawesi Province, Indonesia. AM Fungi spores were isolated and identified based on their morphological characteristics (shape, size, color, attached hyphae, and spore ornamentation). Five types of AMF were identified, namely Glomus aggregatum, Sclerocystis sinuosa, Acaulospora scrobiculata, A. tuberculata, Scutellospora pellucida, and two genera that had not been identified up to species, Glomus sp. and Scutellospora sp. In addition to spores, the symbiosis of AM Fungi with adaptive plants was also demonstrated by the structure of AM Fungi in plant roots with a rate of 74% colonization. S. pellucida and S. sinuosa were recorded as new species in Indonesia and Sulawesi, respectively. While A. tuberculata is a type of AMF that enriches AMF diversity in Southeast Sulawesi.

Based on this study, kayu kuku is categorized as excluder species (TF<1) and moderate species toward Ni (>50 mg Ni/kg of plant dry weight) and possessed very high dependence on AMF (MIE >75%). Local AMF are potential to be developed as biological fertilizer to improve planting stocks for rehabilitation of degraded land.

Kalapi (Kalappia celebica Kosterm.) is an endemic species of Sulawesi and listed in the IUCN red list as an endangered species. In addition, information about silviculuture of K. celebica type in the world is still limited. One important component of silvicultural techniques to support conservation efforts of K. celebica is the application of fertilizers such as mycorrhizal biofertilizers. The aim of the study was to examine the improvement of initial growth of K. celebica by inoculation of arbuscular mycorrhizal fungi (AMF) on gold tailings media. This research was conducted in the greenhouse of the Indonesian Mycorrhizal Association (AMI) branch of Southeast Sulawesi in March - July 2019. The study was designed by using a completely randomized design with 4 AMF inoculums treatments namely control, Acaulospora delicata, Ambiospora appendicula, and Mixed AMF types. Each treatment was repeated 3 times with 5 plant units of each treatment. The results showed that the AMF colonization ranged from 42.2 to 74.6%. The colonization of AMF significantly increased the early growth of K. celebica aged 4 months. Inoculation of A. delicata significantly increased shoot height, number of leaves, shoot and total dry weight. There was no difference of AMF effect in both diameter of the stem and roots dry weight. The dependence of K. celebica on mycorrhizal fungi was high about 51-71% (Mycorrhizal inoculation effect). AMF inoculation improves the initial growth of K. celebica, therefore, it can contribute to the conservation of tropical endangered tree species.

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