No abstract

No abstract

The relation between nutrition and health has been unequivocally established. Wrong eating habits and losses of nutritional factors during technological processing, storage, and culinary treatments can lead to the diet deficient in some nutritional factors. Barley green matter was analysed for the contents of vitamin C, total polyphenols, phenolic compounds, proteins, amino acids, and saccharides; the activity of catalase was also determined. The contents of vitamin C, total polyphenols, and ferulic acid decreased with the age of barley plants. The influence of the variety has not been proved unequivocally. The contents of vitamin C between 0.107-6.357 g/kg DM, of total polyphenols between 17.167-35.559 g/kg DM, and of ferulic acid between 0-5.916 g/kg DM were found. Catalase activity amounted to 4.5-29.7 TSU. The monosaccharide profile showed high contents of glucose (15.40-88.40 g/kg DM) and fructose (37.60-81.40 g/kg DM) which decreased with the plant growth. The contents of saccharose and galactose were low, ranging between 0-7.70 g/kg DM and 3.70-5.30 g/kg DM, respectively. The relations between their contents and the growth phase were insignificant. The total amino acid content decreased with the plant age. High contents of aspartic (15.232-28.682 g/kg DM) and glutamic acids (16.694-35.526 g/kg DM), as well as minimal contents of sulphur amino acids, especially methionin (2.586-5.03 g per kg DM), could be noted. The highest catalase activity was found in the early growth phase (18.5-35.1 TSU), being higher in all samples grown at the location Kroměříž. The yield of juice pressed out from frozen green matter amounted to 68%. The pressed out juice was preserved by fluid drying, freeze drying, and freezing. In respect to folates and total polyphenols contents and the antioxidant activity, freezing appears the most suitable procedure for preserving.

A method based on the polymerase chain reaction (PCR) principle was validated for detecting cow's milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spin Food I by Invitek Co., designed for the samples of animal origin. The PCR method applied utilizes the sequence of the mitochondrial gene coding cytochrome b which is specific for mammals. It uses the common forward primer and the reverse primer species-specific. After electrophoresis, cow DNA was characterised by the fragment of the size of 274 bp, goat DNA by the fragment of 157 bp, and sheep DNA by the fragment of 331 bp. The detection limit of the PCR method described (1%) was determined with model samples made from pure goat cheese with a defined addition of cheese made from cow's milk. The method validated was applied in the analysis of 17 goat cheeses and 7 sheep cheeses obtained from retail trade. Products of Czech, Slovak, French, Dutch, and Italian origin were examined. The presence of undeclared cow's milk was detected in three kinds of goat cheese and in one of sheep cheese.

Green biomass of young barley plants exhibited statistically significant higher activity of superoxide dismutase (SOD) and catalase (CAT) at sampling I (in the phase of plant development DC 29) compared to the later sampling II (DC 31). Significant effects of varieties, years and interactions of the studied factors on the activity of the studied antioxidants were determined. During the experiment period (2005-2007), the variety Sebastian provided statistically significant higher average SOD activity (486 U.g-1) versus the variety Malz (416 U.g-1 dry matter) and line KM1910 (418 U.g-1 dry matter). No statistically significant difference was recorded between the latter two varieties. Average catalase activity of the varieties did not show any significant difference. Significantly higher CAT activity in the sampling I was recorded on average of years and locations in the variety Sebastian and hull-less line KM1910 (935 and 907 U.g-1) compared to the variety Malz (675 U.g-1). We can state that green biomass of young spring barley plants taken during the growth phase DC 29 was a significant source of enzymes catalase and superoxide dismutase in the course of the experiment (2005-2007).