A simple migration test and a more complicated simulation of children's sucking and biting was used to establish migration of DEHP from PVC child-use articles into saliva and compared to a mimic test. The static migration test of a film containing approximately 30% DEHP with saliva simulant gave the lowest values of DEHP; simple shaking increased the amounts of DEHP from 25 +/- 8 to 499 +/- 153 micrograms/g film. The more complicated arrangements to simulate sucking and biting are not so suitable for standard applications. When comparing the in vitro migration tests with the mimic test the factors were 25 and 29 for the static tests (3 and 6 hours) and 1.4 for the shaking test.

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In four groups of patients with chronic conditions receiving an ozone application (intravascular administration of ozone/oxygen mixtures with or without autohemotherapy), the immunoglobulins IgA, IgG and IgM were determined in serum; in groups 1 and 2, lysozyme and vitamin A values also were measured. The groups were broken down as follows: