A four-week study was conducted to determine the effects of feeding rice milling waste (RMW) and supplementary enzyme (Roxazyme G2 ®) on the performance of broiler chicks. One hundred and twenty (120) 7-day old broiler chicks of Anak strain were randomly divided into eight groups of 15 birds each. The groups were randomly assigned to 8 isocaloric (2.85 Mcal of ME/kg) and isonitrogenous (22.00% crude protein) diets in a 4 × 2 factorial arrangement involving a control (0%), three levels (10, 15 and 20%) of RMW and two enzyme levels (0 and 0.02%). Each treatment was replicated three times with five birds per replicate. Results show that feed intake, average daily weight gain, protein efficiency ratio, costs of daily feed intake and feed cost per kg weight gain were significantly (P<0.05) different among the treatment diets. Haematological values such as Haemoglobin concentration (Hb), mean cellular volume (MCH), mean cellular haemoglobin concentration (MCHC) and mean cell volume (MCV) were not significantly (P>0.05) affected by the treatments. Enzyme supplementation resulted in a significant (P<0.05) reduction in feed intake and enhanced significantly (P<0.05), the performance of birds that consumed such enzyme supplemented diets. It was concluded that up to 20% RMW can be included in broiler starter diet without any adverse effect on growth performance of birds. However, the significant increase in feed cost per kg weight gain emanating from the inclusion of enzyme in some of the diets may negate the positive effect that Roxazyme G2 ® enzyme had on growth performance of the broiler chicks.

A prerequisite for successful in vitro culture is the establishment of an aseptic technique, thus the experiment was to investigate suitable sterilization regimes for the leaf explants of Gnetum africanum, an endangered green leafy vegetable. Three sterilization regimes were tested to establish the best regime using three to four days old leaves. The surface sterilized explants were later aseptically introduced onto the surfaces of sterile Murashige and Skoog agar media, incubated at 25°C for three weeks in the growth chamber. 100% sterility was observed from the regime which was significantly different (P<0.05) from the other two regimes thus the best regime adopted for further experiments was; washing in two drops of Tween 20/100 mls of sterile water, soaking in 70% ethanol for 2 min and later in 1% sodium hypochlorite for 20 min. Fungal contaminants responsible for in vitro contaminations was also investigated and possible isolates were identified as Asperigilus niger (28.71%); A. flavus (26.73%); Rhyziopus spp. (24.75%) and Mucor Spp (19.81%) respectively.