The aim of this study was to investigate the occurrence of diarrheagenic Escherichia coli (DEC) strains in raw kibbe samples. For this purpose, 70 samples of raw kibbe were collected from retail establishments and analyzed. Isolated bacterial strains that presented a biochemical profile of E. coli were screened by multiplex PCR for the genetic markers defining the main DEC pathotypes. Two strains belonging to O125:H19 and O149:H8 serotypes were positive for stx 1c genetic sequence and expressed the gene, being thus classified as Shiga-toxigenic E. coli (STEC). These strains were further characterized in respect to other virulence traits and susceptibility to antimicrobial agents. One of them presented the genes astA and lpf O113 and the other harbored only lpf O113. One of the strains was susceptible to all the antimicrobials tested but the other presented an intermediate profile of susceptibility to ampicillin. To the best of our knowledge this is probably the first description of O125:H19 and O149:H8 STEC serotypes in refrigerated raw kibbe and foods in general.
The data presented herein confirm the foodborne disease outbreak. They also allowed for the identification of the source of infection, and suggest that products from poultry are potential reservoirs for this serotype, reinforcing the importance of warning consumers about the danger of possible contamination.
A pesquisa de Pseudomonas aeruginosa foi realizada em amostras de água tratada utilizada na solução de diálise de oito Unidades de Terapia Renal Substitutiva (UTRS) em quatro municípios do noroeste do estado de São Paulo e o perfil de resistência aos agentes antimicrobianos das cepas isoladas foi avaliado por teste de sensibilidade (TSA). Entre 2000 e 2009 foram analisadas 508 amostras seguindo-se a metodologia descrita no Standard Methods for the Examination of Water and Wastewater (2005) e 43 (8,5%) evidenciaram contaminação por P. aeruginosa. Dessas amostras, 55,8%, 23,3% e 20,9% foram, respectivamente, oriundas da sala branca, ponto de pós-osmose/ deionizador e sala amarela das diferentes UTRS. A frequência de isolamento da bactéria foi maior em amostras da UTRS A (53,5%) e em 2004 houve a detecção de maior número de amostras contaminadas (16,7%). Dos 43 isolados, 38 foram analisados pelo TSA, sendo identificadas resistência intermediária a gentamicina em um (2,6%) e resistência a aztreonam e ticarcilina/ácido clavulânico em outro isolado (2,6%). Pelas implicações da P. aeruginosa em saúde pública, sua investigação no monitoramento de qualidade da água para diálise constitui medida necessária e sua ocorrência indica as possíveis deficiências no controle da rede de distribuição da água.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.