Objective: Preeclampsia (PE) is a pregnancy-specific complication defined by the new onset of hypertension and proteinuria during the second trimester of pregnancy. The pathogenesis of PE remains poorly understood. Revealing the key factors involved in placental dysfunction is critical for the understanding the pathogenesis of PE. The aim of this study was to determine the expression levels of ADAMTSs and their molecular partners, TIMP-3 and HAPLNs in the placental tissues of women with PE. Materials and Methods: Experimental research was conducted on control and preeclamptic placentas. A total of 10 control and 10 preeclamptic placentas were included in the present study. The expression levels of ADAMTSs, HAPLNs, and TIMP-3 were analyzed in two groups by Western blot. Results: The expression levels of ADAMTS-4,-8,-10,-12,-13,-14,-16, and-19 were considerably lower, whereas the expression levels of HAPLN-1,-2, and-4; ADAMTS-18; and TIMP-3 were significantly higher in preeclamptic placentas than in controls. Conclusion: Altered expression levels of ADAMTSs and their molecular partners, TIMP-3 and HAPLNs, may contribute to the pathogenesis of PE.

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The lactic acid bacteria are known to be probiotic and their important role has been known in making the biotechnological products and improving human health. Effective probiotic should be viable, safe, bile and gastric juices tolerant, able to survive throughout the human gastrointestinal tract and to colonize a specific human tract. We aimed to investigate and compare the probiotic potential of the Lactobacillus helveticus ATCC 15009 (L. helveticus), Lactobacillus plantarum ATCC 14917 (L. plantarum), Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 (L. bulgaricus), Lactococcus lactis IL1403 (L. lactis IL1403) and Lactococcus lactis IL1403 bearing pSIP plasmid (L. lactis IL403 pSIP) in different aspects that include their ability to tolerate acidic conditions, gastric acid and intestinal juice. We also evaluated their hydrophobicity and antibiotic sensitivity. The Lactobacillus and Lactococcus species were grown at 37 °C and 30°C respectively in different pH, simulated gastric acid and intestinal juice. Four of the bacteria displayed good probiotic features in low pH. Even though L. lactis IL1403 did not survive in L. pH 4.0. Among the tested organisms, cell surface hydrophobicity of L. helveticus was recorded as 79.80±0.008 at Xylene as a highest value. We also found that all bacterial strain could attain to the large intestinal area after 25 hours and are sevsitive to rifampin, chloramphenicol, gentamycin, penicillin and neomycin. Our results suggest that these strains can confer good probiotic but they need to use considering their specific features in accord with therapeutic and biotechnological purposes.

SUMMARY OBJECTIVE: This study aimed to investigate the expression levels of sirtuin 2 and sirtuin 7 in the placenta accreta spectrum to reveal their role in its pathogenesis. METHODS: A total of 30 placenta accreta spectrum, 20 placenta previa, and 30 controls were experienced. The sirtuin 2 and sirtuin 7 expression levels in the placentas of these groups were determined by Western blot. sirtuin 2 and sirtuin 7 serum levels in the maternal and fetal cord blood were examined by enzyme-linked immunosorbent assay. RESULTS: It was found that sirtuin 7 in placenta accreta spectrum was significantly lower in the placenta compared to the control and placenta previa groups (p<0.05). However, a significant difference was not observed between the sirtuin 2 and sirtuin 7 levels in the maternal and fetal cord serum samples of those three groups (p>0.05). CONCLUSION: Sirtuin 7 may play an important role in the formation of placenta accreta spectrum. The effect of decreased expression of sirtuin 7 might be tissue-dependent in the placenta accreta spectrum and needs to be investigated further.