Background and aims: Diabetes mellitus (DM) is a multifactorial metabolic disorder characterized by hyperglycaemia caused by insulin deficiency or insulin resistance. It is a global public health problem. This study aimed to determine specific pharmacological effect of quercetin in water soluble and liposomal preparations in experimental diabetes mellitus. Material and methods: We examined the effect of Corvitin and Lipoflavone (at the dose of 10 mg / kg body weight) in a comparative study in white rats with type 1 diabetes and type 2 diabetes coupled with obesity. To simulate the forms of diabetes mellitus most analogous to those in humans we used Streptozotocin at the doses of 30 mg / kg and 50 mg / kg. We tested the levels of glucose, glycosylated hemoglobin, C-reactive protein, and interleukins 6 and 4 in the blood. Results: In animals with type 1 and type 2 diabetes Lipoflavone significantly reduces glucose and glycosylated hemoglobin levels compared to the rats treated with Corvitin. When administered to animals with diabetes, the effect of quercetin in liposomal form on the concentrations of IL-6, IL-4 and Creactive protein is also larger compared to the water-soluble form. Conclusions: Water soluble quercetin preparation Corvitin and to a larger extent liposomal preparation of this flavonoid, Lipoflavone, show anti-inflammatory effect and restore key parameters of carbohydrate metabolism in experimental type 1 diabetes mellitus and type 2 diabetes coupled with obesity, reducing blood glucose and glycosylated hemoglobin levels.

Background and aims: The prevalence of diabetes mellitus is becoming an epidemic. The diversity of etiological factors contributes to the fact that both types of diabetes mellitus occur among different age groups and among different segments of the population. Activation of various types of processes that lead to oxidative stress, endothelial dysfunction, the development of atherosclerotic changes, increases the risk of macro- and microvascular complications in diabetes mellitus. Material and methods: The experiments were performed on 172 white Wistar rats weighing 120–150 g. All animals were kept on a standard I. Horbachevsky Ternopil National Medical University (TNMU), vivarium diet. In our studies, for the first time was established a distinct cardioprotective effect of quercetin-containing drugs, Corvitin and Lipoflavon, for the purpose of normalizing disorders in type 1 and type 2 diabetes mellitus coupled with obesity, which opens wide opportunities for the introduction of these drugs into diabetic cardiomyopathy. Results: The results obtained are an experimental rationale for extending the indications of clinical use of Corvitin and Lipoflavon. Conclusion: Lipoflavon in the experimental type 1 and type 2 diabetes mellitus coupled with obesity significantly outperforms Corvitin in terms of TBA-active products and diene conjugates.

Aim: Cancer is one of the leading causes of death in the world. The aim of this research was to study the indices of pro- and antioxidant systems in rats with dimethylhydrazine (DMH)-induced colon carcinogenesis on the background of the enterosorbent AUT-M use. Materials and methods: The study was performed on 70 white male rats weighing 200–250 g. Adenocarcinoma of the colon was simulated by subcutaneous injection of the DMH (Sigma-Aldrich Chemie, Japan) at a dose of 7.2 mg/kg once a week during 7 months. Enterosorbent AUT-M was administered intragastrically daily for 21 days after simulation of carcinogenesis at a dose of 1 ml of suspension per 100 g of animal body weight. The state of the pro- and antioxidant systems was studied by the content of oxidative modification of proteins products (OMP), the activity of superoxide dismutase (SOD), catalase (CAT), contents of ceruloplasmin (CP) and reduced glutathione (GSH). Results: It was found that DMH-induced colon carcinogenesis in rats is accompanied by disorders in the antioxidant defense system and activation of free radical oxidation processes. Enterosorbent AUT-M provides a significant reduction in the content of OMP370 and OMP430 in both blood serum and liver homogenate of rats. Moreover, the use of enterosorbent AUT-M demonstrated a significant increase in the activity of SOD, CAT, content of GSH and a decrease in CP content in investigated tissues. Conclusion: The use of enterosorbent AUT-М demonstrated prominent potential suppression for oxidative stress and positive effect on antioxidant defense system in rats with DMH-induced colon carcinogenesis.

Burn wound treatment issues remain relevant in modern medicine, since burns are among the most common types of injuries and rank third among all types of injury in peacetime. The problem of treatment of burn injuries are determined by the high frequency of their occurrence, the complexity and duration of treatment of such patients, with serious consequences. To date, in the world there are a large number of drugs for local treatment of burns in various forms (ointments, gels, creams, aerosols, solutions for external use, patches) and biological drugs are also used. The aim of the work was to conduct a comparative analysis of the range of registered medicinal products for local treatment of burns on the national market, with preparations of this segment of the market, registered in Georgia and France. Georgia is a country, which is not a member of the European Union, and France is one of the five countries leading exporters of medicines and is a member of the European Union, in order to determine the relevance and feasibility of developing new drugs. In the analysis of drugs registered in the pharmaceutical markets of France and Georgia, those drugs that have no analogues in Ukraine, for the most part are patches (patches), considering in more detail. The study of the nomenclature assortment of drugs carrying out in accordance with the State Register of drugs and the Anatomical-Therapeutic-Chemical Classification, as an information source was used Compendium, online-directories and online-pharmacу. According to the results of the research conducted on the end of 2018, in Ukraine there are about 105 drugs, production in various forms that is directly used for local treatment of burns. In the pharmaceutical markets of Georgia and France, this amount of drugs is respectively 101 and 64 drugs. In all three countries, most medications for local treatment of burns presented in soft drugs, in particular, in Ukraine 67.58%, in Georgia 84.16% and in France 39.06%, respectively. In Ukraine, 58.33% of all drugs for local treatment of burns domestically produced, but in Georgia and France, the proportion of such drugs is significantly lower, namely 11.88% and 32.81% respectively. The comparative analysis conducted by us proved that in the pharmaceutical markets of France and Georgia there were registered drugs in the form of patches that are widely used in the treatment of burn injuries, the analogues of which are absent in Ukraine. These studies prove the prospect of expanding the range of existing ursus forms and creating a new group of original drugs that would stimulate regenerative skin processes and improve wound healing.

The problem of studying the metabolic syndrome, as well as its integration into other pathological processes, despite large-scale research, remains relevant. The complexity of the interaction of different links in pathogenesis requires scientists to find new tools and methods for both diagnosis and treatment. Drone brood homogenate, which is a multifactorial pharmacological agent in terms of chemical composition, seems to be promising to study for today. And the lack of contraindications and a wide age range makes it an excellent object of research. The current study evaluated the pharmacological aspects of safety: acute toxicity, effects on the functional and motor activity of the gastrointestinal tract, as well as local irritation of the gastric mucosa, the secretory function of the stomach. All experiments were performed according to the classical methods. The specific pharmacological activity of the drone brood homogenate was determined in comparison with metformin in the experimental fructose metabolic syndrome. Animals obtained from the Vivarium of I.Horbachevsky Ternopil National Medical University were used to implement the set goals. Working with animals was met all bioethical requirements. The study found that the lyophilized drone brood homogenate does not have a local irritant effect and does not cause ulcers on the surface of the gastric mucosa, does not affect the secretory function of the stomach and motor-evacuatory activity of the gastrointestinal tract and is a low-toxic substance, indicating the possibility of its long-term safe use. As expected, glucose, insulin, and HOMA index were significantly increased in animals that were simulated metabolic syndrome. The use of drone brood homogenate by animals contributed to a relatively positive effect on selected indicators of the metabolic syndrome. Accordingly, drone brood homogenate is a promising active pharmaceutical ingredient for the normalization of biochemical disorders in metabolic syndrome.