BackgroundDespite the increasing number of reports of autochthonous cases of ocular thelaziosis in dogs in several European countries, and the evident emergence of human cases, the distribution and spreading potential of this parasite is far for being fully known. In Romania, despite intensive surveillance performed over recent years on the typical hosts of T. callipaeda, the parasite has not been found until now.MethodsIn October 2014 a German Shepherd was presented for consultation to a private veterinary practice from western Romania with a history of unilateral chronic conjunctivitis. Following a close examination of the affected eye, nematodes were noticed in the conjunctival sac. The specimens collected were used for morphological examination (light microscopy) and molecular analysis (amplification of the cytochrome c oxidase subunit 1 gene, followed by sequencing).ResultsThirteen nematodes were collected, all identified morphologically as T. callipaeda. The history of the dog revealed no travel outside Romania, and during the last year, not even outside the home locality. The BLAST analysis of our sequence showed a 100% similarity T. callipaeda haplotype h1.ConclusionsThis is the first report of T. callipaeda in Romania, which we consider to be with autochthonous transmission. These findings confirm the spreading trend of T callipaeda and the increased risk of emerging vector-borne zoonoses.

Background The aim was to assess the effects of periodontal disease in promoting liver fibrosis in a rat model of ligature‐induced periodontitis. Methods Twenty‐four Wistar rats were divided into four groups: control (CTRL), experimental periodontitis group at day 7 (PER7), at day 14 (PER14), at day 21 (PER21). Experimental periodontitis was induced by the placement of a silk ligature around mandibular incisors. The following parameters were assessed: gingival index, tooth mobility; liver status, and portal vein caliber by ultrasound examination; bone retraction, bone mineral density (BMD), bone volume/tissue volume (BV/TV) by micro‐CT analysis; aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT); oxidative stress (malondialdehyde [MDA], reduced glutathione/oxidative glutathione ratio [GSH/GSSG]), and matrix metalloproteinase‐8 (MMP‐8) levels; and histopathological evaluation of periodontal and liver tissues. Results Periodontal parameters showed the development of periodontitis in experimental groups. Micro‐CT results indicates an increase of bone retraction and BMD values and a decrease of BV/TV value in PER groups. Liver fibrosis could not be diagnosed with ultrasound examination in any of the groups. Elevated levels of ASAT and ALAT in PER groups compared with CTRL group were found. MDA have indicated elevated levels and a decrease of GSH/GSSG ratio in PER group compared with the CTRL group. Levels of MMP‐8 have indicated high values in PER21 compared with the other groups. Histological analysis of the periodontal and liver tissues sustains the link between periodontal and hepatic injury. Conclusion This study demonstrates a positive correlation between periodontal lesions and liver disease. Periodontitis may be an independent risk factor for liver fibrosis.

Despite recent advances in disease management and prevention, heart failure (HF) prevalence is still high. Hypertension, inflammation and oxidative stress are being investigated as important causative processes in HF. L. barbarum L. polysaccharides (LBPs) are widely used for their anti-inflammatory and antioxidant properties. Thus, the aim of the present study was to evaluate the effects of LBPs on inflammation and oxidative stress markers in a pressure overload-induced HF rat model, surgically induced by abdominal aorta banding in Wistar rats (AAB) (n = 28). Also, control rats (n = 10) were subjected to a sham operation. After echocardiographic confirmation of HF (week 24), AAB rats were divided into three groups: rats treated with LBPs for 12 weeks: 100 mg/kg body weight /day (AAB_100, n = 9), 200 mg/kg body weight /day (AAB_200, n = 7) and no-treatment group (control AAB, n = 12). After 12 weeks of treatment with LBPs, the decline of cardiac function was prevented compared to the control AAB rats. Treatment with 200 mg/kg body weight /day LBPs significantly reduced the inflammation as seen by cytokine levels (IL-6 and TNF-α) and the plasma lipid peroxidation, as seen by malondialdehyde levels. These results suggest that LBPs present anti-inflammatory and antioxidant effects with utility in a HF animal model and encourage further investigation of the cardioprotective effects of these polysaccharides.

In modern veterinary medicine, like in human medicine all major surgery procedures must be performed under balanced anaesthesia. This protocol contained substances that insure: neurovegetativ protection, analgesia, miorelaxation, hypnosis. Rocuronium represent a nondepolarizing agent, intermediate acting ( 60-120 seconds), with relative long effects ( 40-90 minutes), and with the posibility of effect reversal ( agents-Sugammadex). The aim of this study was monitorization of clinical, haematological and biochemical parameters perioperative in dogs scheduled for orthopedic procedures. This study was performed on ive canine patients (different breed, with age between 4 months to 11 years), scheduled for femoral had resection procedure. In this dogs balanced anaesthesia was performed: induction with propofol to effect, maintained with sevo lurane 2.5%; analgesic component: fentanyl 3μg/kg b.w.-bolus followed by CRI 5 μg/kg b.w./min, the neuromuscular blocking agent was represented by rocuronium 0.4 mg/kg b.w. Haematological analysis were performed with Abacus Junior Vet 3 diff analyzer, biochemical parameters with UV-VIS Screen Master Touch Hospitex Diagnostic spectrophotometer, and electrolytes with GEM Premier 2000 analyzer. The majority of biochemical and mineral parameters suffered only small changes, without statistical importance. After the surgery we determined only a slight reduction of erythrocyte number, haemoglobin and PCV, a mild increase of γ-globulin. Regarding the clinical parameters only internal temperatures was modi ied. We recommend the use of rocuronium in orthopedic procedures for the advantages offered in the surgical act (profound miorelaxation) without any major biochemical, haematological, electrolytes alteration.

A survey with 32 questions was performed in Cluj-Napoca veterinary private practices about the routine anaesthetic management of dogs. Of those veterinarians who answered this questionnaire, 18.2% are equipped with a machine for inhalation anesthesia, 27.3% do not use any monitoring during anesthesia and no one use methods of monitoring blood pressure. All the veterinarians graduated the Faculty of Veterinary Medicine Cluj-Napoca.  Ketamine and alpha 2 agonists remain the most used anesthetic substances in private practices. Postoperative analgesia is used constantly by 81.8% of veterinarians. The mortality rate in veterinary practices in Cluj - Napoca for two years was 0.25% (1:403).