Three-dimensional (3D) cultures models may more accurate representation of the in vivo environment than two-dimensional (2D) cultures while maintaining the cytoarchitecture of in situ tissue that supports cells differentiation or maturation [1, 2]. Cell adhesion is depended upon surface hydrophilicity, surface charge density, surface morpho logy, specific chemical groups present on the surface of the scaffold [3]. Given that surface chemistry is crucial for the biocompatibility of the nanolayers, specific surface modifications are used with different polymers [4, 5]. In particular, there is an increased interest in the polymeric surfaces which can change their affinity towards proteins and cells under external stimuli [6, 7] and therefore have potential applications in biology and medicine. Despite various investigations, specific and complex mechanisms govern the reactions that occur between the biomaterial and the cellular environment are still incomplete understanding. The objective of this study was to establish and comparison cells line B16F10 viability cultured onto different coatings. We used surfaces obtained by grafting APTES, dextran, albumin and their combinations to the surface of the modified glass plates. Materials and Methods Preparation of coatings. Glass plates (2020) were dipped into 0.2 % (w/w) methanolic solution of (3-aminopropyl)triethoxysilane (APTES) for 24 h. After the incubation, loosely-attached silane molecules were removed with methanol in Soxhlet's apparatus. Then the plates functionalized with APTES

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In reproductive medicine it is important to determine the quality of embryo development without interference in their function and viability. The surface plasmon resonance of silver nanoparticles makes them promising candidates for optical sensing, molecular labeling and imaging applications. Furthermore unique optical properties of silver nanoparticles provide an opportunity to use them as real time analytic tools in living state especially for observation of dynamic processes in gametes and embryos. The main aim of the study was to investigate the physicochemical properties and biological activities of novel silver nanoparticles with prospect of their use for the determining the quality of embryo development. For this purpose, we investigated the optical properties of new silver nanoparticles in biological mediums during cultivation and their influence on rabbit's embryos development in vitro. The physicochemical and biological properties of novel silver nanoparticles were compared with silver nanoparticles identical in size and shapes but with different chemical surfaces modifications by polyvinylpyrrolidone and bovine serum albumin. The results suggest that silver nanoparticles with hyaluronic acid were disintegrated with the formation of new complexes with proteins in biological mediums. This property with strong optical surface plasmon resonance of novel silver nanoparticles with hyaluronan makes them promising candidates in diagnostic area and gives reasons to explore them as biomarkers of target molecules. Nevertheless novel silver nanoparticles with hyaluronan at the concentrations of 0.1-1 µg/ml have no toxic effect on rabbit's embryos development and can be successfully applied in reproductive medicine.

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