Three-dimensional (3D) cultures models may more accurate representation of the in vivo environment than two-dimensional (2D) cultures while maintaining the cytoarchitecture of in situ tissue that supports cells differentiation or maturation [1, 2]. Cell adhesion is depended upon surface hydrophilicity, surface charge density, surface morpho logy, specific chemical groups present on the surface of the scaffold [3]. Given that surface chemistry is crucial for the biocompatibility of the nanolayers, specific surface modifications are used with different polymers [4, 5]. In particular, there is an increased interest in the polymeric surfaces which can change their affinity towards proteins and cells under external stimuli [6, 7] and therefore have potential applications in biology and medicine. Despite various investigations, specific and complex mechanisms govern the reactions that occur between the biomaterial and the cellular environment are still incomplete understanding. The objective of this study was to establish and comparison cells line B16F10 viability cultured onto different coatings. We used surfaces obtained by grafting APTES, dextran, albumin and their combinations to the surface of the modified glass plates. Materials and Methods Preparation of coatings. Glass plates (2020) were dipped into 0.2 % (w/w) methanolic solution of (3-aminopropyl)triethoxysilane (APTES) for 24 h. After the incubation, loosely-attached silane molecules were removed with methanol in Soxhlet's apparatus. Then the plates functionalized with APTES

The more stable among the tested samples were samples with saccharose in the concentration of 50–75 mg per cm3. While adding of L- lysine to samples the most stable activity was discovered in the experimental series of samples with the content of lysine of 10 mg per cm3 – activity increased by 54 % as compared to theoretical initial activity of HCG during 8 weeks. While storing gonadotropin with L-glycine fluctuations of hormone activity in all series of samples were observed. Adding of 0.2 mg per cm3 of L-glycine had a more expressed stabilizing effect. Adding of 0.2 mg per cm3 of L-methionine produced relatively high and stable activity of gonadotropin during the 6 weeks storage. Adding of 0.25 mg / cm3 of L- glycine and 75.50 mg / cm3 of saccharose to experimental samples during 2 weeks at 40 °C provided 69.8 % and 60.7 % saving activity of hCG respectively. Activity of gonadotropin in a series of samples with the addition of L- glycine and mannitol was significantly lower and at the end of the study was at an appropriate rate with the control series models. The highest activity of gonadotropin was detected while adding fillers – 10 mg / cm3 L-lysine and 75 mg / cm3 saccharose and mannitol – to recipes as a stabilizer.