Plant extracts rich in phenolic compounds have been demonstrated to accelerate wound healing, but their use by oral route has been poorly studied. The leaves of Vitis labrusca are rich in phenolic acids and flavonoids. The goal of this study was to assess the healing properties of the oral administration of hydroalcoholic extract of V. labrusca leaves (HEVL) in a murine model. HEVL was obtained by Soxhlet and dynamic maceration, and their yield and phenolic acids and flavonoid contents were determined. For the wound healing assay, 8 mm wounds were performed on the back of 48 Wistar rats, assigned into four groups (n = 12): CTR (distilled water), HEVL100, HEVL200, and HEVL300 (HEVL at 100, 200, and 300 mg/kg, respectively). On days 7 and 14, wound closure rates were assessed, and the healing wounds were subjected to histological analysis. Soxhlet-obtained extract was selected for the wound healing assay because it provided a higher yield and phenolic acid and flavonoid contents. HEVL significantly reduced leukocytosis in the peripheral blood (p < 0.05), accelerated wound closure (p < 0.05), and improved collagenization (p < 0.05) on day 7, as well as enhanced the epidermal tissue thickness (p < 0.001) and elastic fiber deposition on day 14 (p < 0.01). Furthermore, HEVL promoted an increase in the histological grading of wound healing on both days 7 and 14 (p < 0.01). The doses of 200 and 300 mg/kg provided better results than 100 mg/Kg. Our data provide histological evidence that the oral administration of HEVL improves wound healing in rodents. Therefore, the extract can be a potential oral medicine for healing purposes.

The Himatanthus genus presents anti-inflammatory, antioxidant activities, suggesting potential wound-healing properties. This study aimed to develop and analyze the wound-healing properties of a photopolymerizable gelatin-based hydrogel (GelMA) containing an ethanolic extract of Himatanthus bracteatus in a murine model. The extract was obtained under high pressure conditions, incorporated (2%) into the GelMA (GelMA-HB), and physically characterized. The anti-inflammatory activity of the extract was assessed using a carrageenan-induced pleurisy model and the GelMA-HB scarring properties in a wound-healing assay. The extract reduced IL-1β and TNF-α levels (48.5 ± 6.7 and 64.1 ± 4.9 pg/mL) compared to the vehicle (94.4 ± 2.3 pg/mL and 106.3 ± 5.7 pg/mL; p < 0.001). GelMA-HB depicted significantly lower swelling and increased resistance to mechanical compression compared to GelMA (p < 0.05). GelMA-HB accelerated wound closure over the time course of the experiment (p < 0.05) and promoted a significantly greater peak of myofibroblast differentiation (36.1 ± 6.6 cells) and microvascular density (23.1 ± 0.7 microvessels) on day 7 in comparison to GelMA (31.9 ± 5.3 cells and 20.2 ± 0.6 microvessels) and the control (25.8 ± 4.6 cells and 17.5 ± 0.5 microvessels) (p < 0.05). In conclusion, GelMA-HB improved wound healing in rodents, probably by modulating the inflammatory response and myofibroblastic and microvascular differentiation.

Em meados de dezembro de 2019, surgiram os primeiros casos da doença causada pelo novo Coronavírus 2019, o SARS-CoV-2, na cidade de Wuhan (China). A doença causada pelo novo Coronavírus (COVID-19) acomete inicialmente o trato respiratório e seu curso clínico pode acarretar disfunções orgânicas no hospedeiro. Neste ínterim, os exames laboratoriais são ferramentas de diagnóstico de grande importância para a detecção do SARS-CoV-2, confirmação da doença e observação de disfunções sistêmicas. Desta forma, este estudo objetivou revisar as metodologias de diagnóstico do SARS-CoV-2 e alterações nos exames laboratoriais. Trata-se de uma revisão literária realizada entre 01 de janeiro e 31 de maio de 2020 a partir de dados advindos de trabalhos publicados em revistas científicas disponíveis na íntegra. Para tanto, foram utilizadas as bases de dados PubMed, Science Direct e Scielo e os seguintes descritores em inglês: coronavirus, covid-19, sars-cov-2, laboratory medicine, laboratory tests, laboratory findings, RT-PCR, immunochromatography e chemiluminescence.  Foram encontrados 2810 artigos científicos relacionados onde apenas 24 foi considerado para o estudo. O SARS-CoV-2 pode ser detectado em amostras respiratórias através de metodologias de diagnóstico laboratorial como o RT-PCR (Real-time Polymerase Chain Reaction) ou através de detecção de anticorpos através dos testes imunocromatográficos, imunosorventes e quimioiluminescentes. Además, podem ser observadas alterações hematológicas relacionadas ao leucograma, coagulação, marcadores hepáticos e renais bem como marcadores inflamatórios.  Em suma, as ferramentas de diagnóstico têm sido cruciais para o acompanhamento do paciente com sintomas da COVID, servindo de apoio aos clínicos na tomada de decisões.